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CIF

p,td { font-size:14px_!important; text-align:justify; line-height:_19px; margin-bottom:_10px_!important; } Central_Instrumentation_Facility_(CIF)

 

 

 

Technical_Staffs_
SN Name Designation Contact 1. Mr._Ramesh_Bhardwaj Senior_Technical_Assistant    2. Mrs._Rashmi_Sinha Technical_Assistant +91-7065474421  

 

cifsesjnu@gmail.com

011_2670_4562

011_2670_4161

Instruments_

 

1.Atomic_absorption_spectrophotometer_(AAS)
2.X-Ray_Fluorescence_(XRF)
3.X-Ray_Diffraction_(XRD)
4.Spectrofluorophotometer
5.Akta_Fast_protein_liquid_chromatography_(FPLC)
6.Ultracentrifuge
7.Thermal/Optical_Carbon_Analyzer
8.Carl-Zeiss_Microscope
9.Gas_Chromatography_(GC)
10.Flowcytometry
 
Instruments

1.AAS
In_flame_atomic_absorption_spectrometry,_a_sample_is_aspirated_into_a_flame_and_atomized._A_light_beam_is_directed_through_the_flame,_into_a_monochromator,_and_onto_a_detector_that_measures_the_amount_of_light_absorbed_by_the_atomized_element_in_the_flame._Because_each_metal_has_its_own_characteristic_absorption_wavelength,_a_source_lamp_composed_of_that_element_is_used;_this_makes_the_method_relatively_free_from_spectral_or_radiation_interferences._The_amount_of_energy_at_the_characteristic_wavelength_absorbed_in_the_flame_is_proportional_to_the_concentration_of_the_element_in_the_sample_over_a_limited_concentration_range.

 

Application:_Quantification_of_elements_in_water,_wastewater_and_soil_sample,_etc. 

Model:_Termo_AAS_M6_System

Sr._No._651187

Software:_AAS_SOLAAR

  2._XRF
X-ray_fluorescence_(XRF)_is_nondestructive_technique_used_in_quantitative_elemental_analysis_of_a_wide_range_of_organic_and_inorganic_samples._The_basis_for_the_technique_is_that_all_elements_emit_secondary_(‘fluorescent’)_X-rays_of_characteristic_energy_when_exposed_to_X-rays_of_appropriate_higher_energy,_with_energy_and_intensity_of_emitted_X-rays_used_to_determine_elemental_composition._In_general,_the_heavier_the_element_being_analyzed,_the_higher_the_energy_of_X-rays_required_to_elicit_fluorescence,_the_higher_the_energy_of_fluorescence,_and_the_easier_it_is_to_detect_fluorescence._The_lightest_elements_found_in_biological_samples_(e.g._H,_B,_C,_N,_O)_are_not_generally_detectable_by_XRF,_while_elements_such_as_Na,_Mg,_P,_S,_Cl,_K,_Ca_are_detectable,_but_only_at_high_concentrations_or_with_highly_specialized_conditions,_and_heavier_elements_such_as_the_trace_metals_Mn,_Fe,_Cu_and_Zn_or_toxic_heavy_metals_are_readily_analyzed,_even_at_trace_levels._Major_advantages_of_XRF_over_‘wet_chemistry’_methods_are_that_analyses_are,_use_no_noxious_chemicals_and_produce_no_toxic_wastes,_and_can_be_made_on_solid_samples._Since_XRF_signal_is_obtained_from_transitions_among_inner_shell_electrons,_not_bonding_electrons,_XRF_also_has_the_advantage_that_signal_is_independent_of_chemical_form._The_Wavelength-Dispersive_XRF_(WD-XRF),_which_discriminates_emissions_after_diffraction_through_a_crystal._The_major_advantage_of_EDXRF_over_WD-XRF_is_that_equipment_is_simpler_and_cheaper_with_lower_output_X-ray_tubes,_albeit_with_lower_resolving_power_and_higher_background.

 

Model:_PANalytical_WD-XRF_AXIO-1_System

Sr._No._DY2174

Software:_Super-Q

  3._XRD
X-ray_diffraction_is_tool_used_for_identifying_the_atomic_and_molecular_structure_of_a_crystal_in_crystallographic_study,_in_this_the_crystalline_atoms_cause_a_beam_of_incident_X-rays_to_diffract_into_many_specific_directions._By_measuring_the_angles_and_intensities_of_these_diffracted_beams,_a_crystallographer_can_produce_a_three-dimensional_picture_of_the_density_of_electrons_within_the_crystal._From_this_electron_density,_the_mean_positions_of_the_atoms_in_the_crystal_can_be_determined,_as_well_as_their_chemical_bonds,_their_disorder_and_various_other_information.

 

Model:_Philips_Xpert_pro_XRD_System

Sr._No._DY1650

Software:_Super-Q

  4._Spectrofluorophotometer_:
The_spectrofluorophotometer_irradiates_a_sample_with_excitation_light_of_particular_wavelength_from_the_light_using_Xenon_lamp_and_measures_the_fluorescence_emitted_from_the_irradiated_sample_to_perform_a_qualitative_or_quantitative_analysis.

 

Model:_RFPC_Shimadzu

Software:_RF5301PC

  5._Akta_Fast_protein_liquid_chromatography_(FPLC)
It_is_a_form_of_liquid_chromatography_that_is_often_used_to_analyze_or_purify_mixtures_of_proteins._As_in_other_forms_of_chromatography,_separation_is_possible_because_the_different_components_of_a_mixture_have_different_affinities_for_two_materials,_a_moving_fluid_(the_"mobile_phase")_and_a_porous_solid_(the_stationary_phase)._In_FPLC_the_mobile_phase_is_an_aqueous_solution,_or_"buffer"._The_buffer_flow_rate_is_controlled_by_a_positive-displacement_pump_and_is_normally_kept_constant,_while_the_composition_of_the_buffer_can_be_varied_by_drawing_fluids_in_different_proportions_from_two_or_more_external_reservoirs._The_stationary_phase_is_a_resin_composed_of_beads,_usually_of_cross-linked_agarose,_packed_into_a_cylindrical_glass_or_plastic_column._FPLC_resins_are_available_in_a_wide_range_of_bead_sizes_and_surface_ligands_depending_on_the_application.

 

Model:_Akta_FPLC

  6._Ultracentrifuge:
The_latest_in_tabletop_ultracentrifuge_technology._The_OptimaTM_MAX-XP_tabletop_ultracentrifuge_is_a_power-packed_solution_that_delivers_fast,_efficient_separations_from_samples_as_small_as_200_μl_up_to_32.0_ml_and_at_speeds_of_up_to_150,000_RPM_and_more_than_1,000,000_x_g._Every_detail_of_the_Optima_MAX-XP_is_designed_with_the_user_in_mind–from_the_system's_compact_design,_versatility_and_quiet_operation_to_advanced_multi-lingual_software.

 

Model:_Optima_MAX-XP,_Bekman

  7._Thermal/Optical_Carbon_Analyzer
The_operation_of_the_DRI_Model_2001_Thermal/Optical_Carbon_Analyzer_is_based_on_the_preferential_oxidation_of_organic_and_elemental_carbon_(OC_and_EC)_compounds_at_different_temperatures._Its_function_relies_on_the_fact_that_organic_compounds_can_be_volatilized_from_the_sample_deposit_in_a_non-oxidizing_helium_(He)_atmosphere,_while_EC_must_be_combusted_with_an_oxidizer._The_analyzer_operates_by:_1)_liberating_carbon_compounds_under_different_temperature_and_oxidation_environments_from_a_small_sample_punch_taken_from_a_quartz-fiber_filter;_2)_converting_these_compounds_to_carbon_dioxide_(CO2)_by_passing_the_volatilized_compounds_through_an_oxidizer_(heated_manganese_dioxide,_MnO2);_3)_reducing_CO2_to_methane_(CH4)_by_passing_the_flow_through_a_methanator_(hydrogen-enriched_nickel_catalyst);_and_4)_quantifying_CH4_equivalents_with_a_flame_ionization_detector_(FID)._Values_routinely_reported_include_total_OC,_total_EC,_total_carbon_(TC,_sum_of_total_OC_and_total_EC),_and_pyrolyzed_carbon,_monitored_by_both_reflectance_(OPR)_and_transmittance_(OPT)._Depending_on_the_thermal/optical_protocol_applied_for_quantification,_thermally-derived_subfractions_of_OC_and_EC,_and_of_carbonate_carbon,_are_reported.

 

Model:_DRI_2001

Aerosol_Filter_Samples_-_Method_IMPROVE_A_Revision:_3

  8._Carl-Zeiss_Microscope
Carl-Zeiss_microscopes_used_in_applications_of_biology_and_medicine_for_the_examination_of_blood,_tissue_etc.,_specimens,_as_well_as_for_materials_examinations._They_may_also_be_used_as_true_reflected-light_microscopes_or,_if_equipped_with_a_transmitted-light_equipment,_as_combined_reflected-light/transmitted-light_microscopes._Typical_applications_of_the_Axio_Imager_microscopes_include,_for_instance:_Medical_examinations_in_laboratories_(research),_clinics_and_medical_practices._Science_and_research_(colleges,_universities)_in_the_fields_of_medicine_and_biology._Industrial_applications_(pharmacology,_food_technology)

 

  9._Gas_Chromatography_
Gas_Chromatography_is_an_analytical_instrument_used_in_separating_and_analyzing_compounds_that_can_be_vaporized_without_decomposition._The_sample_is_transported_through_column_by_the_flow_of_inert_gaseous_mobile_phase_such_as_helium,_nitrogen,_Argon_&_Zero_air.

Model:_GC-2010,_SHIMADZU

Software:_GC_solution

 

  10._FACS
Flow_cytometry_is_a_laser-or-_impedance_based_biophysical_technology_employed_in_cell_counting,_cell_sorting_and_biomarker_detection._The_BD_FACS_verse_flow_cytometry_system_acquires_and_analyses_particles_or_cells_in_a_liquid_suspension._Antibodies_to_specific_cell_proteins_are_labeled_with_a_fluorescent_dye_and_incubated_with_the_cell_suspension._The_suspension_flows_through_the_cytometer_and_is_interrogated_by_a_laser_which_excites_the_fluorescent_antibodies._The_Fluoresence_is_captured_and_the_resulting_data_is_analyzed_to_reveal_information_about_the_cells.

Make-_BD_FACS_Verse,

Software:_BD_FACSuite