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Faculty Profile

p{ text-align:justify; line-height:_19px; font-size:14px; } li{ text-align:justify; line-height:_19px; font-size:14px; margin-bottom:_5px_!important; } Dr._Chinmay_K._Mukhopadhyay,_Professor_

Prof._Mukhopadhyay_received_his_Ph.D._from_the_Department_of_Biochemistry_from_Calcutta_University._He_received_the_postdoctoral_training_at_the_Department_of_Cell_Biology,_Cleveland_Clinic_Foundation,_USA._He_joined_at_the_‘Special_Centre_for_Molecular_Medicine’_in_2001._Prof._Mukhopadhyay_received_International_Senior_Research_Fellowship_from_The_Wellcome_Trust,_UK._He_is_an_elected_member_of_Guha_Research_Conference_and_Fellow_of_The_National_Academy_of_Sciences._He_is_an_Editorial_Board_Member_in_the_journal_Scientific_Reports_and_Reviewing_Editor_of_Frontiers_in_Physiology._

 

Research_Interests:

Iron_is_essential_micronutrient_for_all_the_organisms_because_of_its_ability_to_function_as_a_protein_bound_red-ox_element._Defective_regulation_of_iron_homeostasis_leads_to_either,_iron_excess_and_related_tissue_injuries_due_to_iron-stimulated_oxidative_damage_or_iron_deficiency_disorders._Alterations_of_iron_pool_are_implicated_in_tissue_injury,_diabetes_and_obesity,_cancers,_neurodegenerative_diseases,_inflammation_and_infections._Prof._Mukhopadhyay’s_research_interest_includes_understanding_the_role_of_iron_in_insulin_resistance_and_related_disorders,_neurodegenerative_diseases_like_Parkinson’s_disease._He_is_also_interested_in_unravelling_mechanisms_by_which_protozoan_parasite_Leishmania_donovani_alters_iron_homeostasis_in_macrophages_for_its_survival_and_growth,_His_recent_research_interest_extends_in_understanding_the_role_of_iron_and_reactive_oxygen_species_in_inflammation_of_macrophages_and_microglia._

 

Ongoing_Projects:

Mukhopadhyay_CK._Understanding_the_role_and_regulation_of_ferroportin_in_reactive_oxygen_species-induced_activation_of_microglia._Science_and_Engineering_Research_Board,_2021-2024_

The_project_aims_to_explore_the_role_of_exogenous_and_endogenous_reactive_oxygen_species_in_regulating_iron_exporter_ferroportin_and_its_role_in_activation_of_microglia.

 

_Mukhopadhyay_CK._Role_of_norepinephrine_on_mitochondrial_iron_homeostasis_in_astroglial_cell._Department_of_Biotechnology._2023-2026_

The_project_aims_to_understand_the_role_of_norepinephrine_on_astroglial_mitochondrial_iron_homeostasis,_ATP_generation_and_release._This_may_be_helpful_in_regulating_ATP_release_from_astroglia_to_modulate_postsynaptic_efficacy_affected_in_several_neurodegenerative_diseases._

 

Collaborations:

Prof._Neena_Singh,_Case_Western_Reserve_University,_USA Prof._Pankaj_Seth,_National_Brain_Research_Centre,_Manesar Prof._Amit_Dinda,_All_India_Institute_of_Medical_Sciences,_New_Delhi_

 

Selected_Publications_

  1. Sen_S,_Bal_SK,_Yadav_S,_Mishra_P,_Vivek_VG,_Rastogi_R,_Mukhopadhyay_CK._Intracellular_pathogen_Leishmania_intervenes_in_iron_loading_into_ferritin_by_cleaving_chaperones_in_host_macrophages_as_an_iron_acquisition_strategy._J._Biol._Chem._298:102646,_2022
  2. Gupta_P,_Singh_P,_Pandey_HS,_Seth_P,_Mukhopadhyay_CK._Phosphoinositide-3-kinase_inhibition_elevates_ferritin_level_resulting_depletion_of_labile_iron_pool_and_blocking_of_glioma_cell_proliferation._Biochim_Biophys_Acta_-_General_Subjects,_1863(3):547-564,_2019
  3. Das_NK,_Sandhya_S,_Vishnu_Vivek_G,_Kumar_R,_Singh_AK,_Bal_SK,_Kumari_S,_Mukhopadhyay_CK.._Leishmania_donovani_inhibits_ferroportin_translation_by_modulating_FBXL5-IRP2_axis_for_its_growth_within_host_macrophages._Cell_Microbiol._20(7):e12834._2018
  4. Dev_S,_Kumari_S,_Singh_N,_Bal_SK,_Seth_P,_Mukhopadhyay_CK._Role_of_extracellular_hydrogen_peroxide_on_regulation_of_iron_homeostasis_genes_in_neuronal_cell:_Implication_in_iron_accumulation._Free_Radical_Biol_Med._86:78-89,_2015.
  5. Tapryal_N,_Vivek_VG,_Mukhopadhyay_CK._Catecholamine_stress_hormones_regulate_cellular_iron_homeostasis_by_a_posttranscriptional_mechanism_mediated_by_iron_regulatory_protein:_Implication_in_energy_homeostasis._J._Biol._Chem._290:7634-7646,_2015
  6. Biswas_S,_Tapryal_N,_Mukherjee_R,_Kumar_R,_Mukhopadhyay_CK._Insulin_promotes_iron_uptake_in_human_hepatic_cell_by_regulating_transferrin_receptor-1_transcription_mediated_by_hypoxia_inducible_factor-1. _Biochim_Biophys_Acta._1832(2):293-301,_2013
  7. Tapryal_N,_Mukhopadhyay_C,_Mishra_MK,_Das_D,_Biswas_S,_Mukhopadhyay_CK._Glutathione_synthesis_inhibitor_butathione_sulfoximine_regulates_ceruloplasmin_by_dual_but_opposite_mechanism:_Implication_in_hepatic_iron_overload._Free_Radic_Biol_Med._48:1492-1500,_2010.
  8. Tapryal_N,_Mukhopadhyay_.,_Das_D,_Fox_PL,_Mukhopadhyay_CK._Reactive_oxygen_species_regulate_ceruloplasmin_by_a_novel_mRNA_decay_mechanism_involving_its_3'-untranslated_region:_Implications_in_neurodegenerative_diseases._J._Biol._Chem._284:1873-1883,_2009.
  9. Das_NK,_Biswas_S,_Solanki_S,_Mukhopadhyay_CK._Leishmania_donovani_depletes_labile_iron_pool_to_exploit_iron_uptake_capacity_of_macrophage_for_its_intracellular_growth._Cell_Microbiol._11:83-94,_2009.
  10. Biswas_S,_Gupta_MK,_Chattopadhyay_D,_Mukhopadhyay_CK._Insulin_induced_activation_of_hypoxia_inducible_factor-1_requires_generation_of_reactive_oxygen_species_by_NADPH_oxidase._Am._J._Physiol._Heart_and_Circulatory_Physiology_292:H758-766,_2007.
  11. Das_D,_Tapryal_N,_Goswami_SK,_Fox_PL,_Mukhopadhyay_CK._Regulation_of_Ceruloplasmin_in_human_hepatic_cells_by_redox_active_copper:_Identification_of_a_novel_AP-1_site_in_ceruloplasmin_gene._Biochem_J._402:135-141,_2007.
  12. Seshadri_V,_Fox_PL._Mukhopadhyay_CK._Dual_role_of_insulin_in_transcriptional_regulation_of_the_acute_phase_reactant_ceruloplasmin._J_Biol_Chem._277:_27903-27911,_2002.
  13. Mukhopadhyay_CK,_Attieh_ZK._Fox_PL._Role_of_ceruloplasmin_in_cellular_iron_uptake. _Science._279:_714-717,_1998.
  14. Mukhopadhyay_CK,_Mazumder_B,_Lindley_PF,_Fox_PL._Identification_of_the_prooxidant_site_of_human_ceruloplasmin:_A_model_for_oxidative_damage_by_copper_bound_to_protein_surfaces._Proc._Natl._Acad._Sci._USA,_94:11546-11551,_1997.
  15. Mukhopadhyay_CK,_Chatterjee_IB._Free_metal_ion-independent_oxidative_damage_of_collagen._Protection_by_ascorbic_acid._J._Biol._Chem._269:30200-30205,_1994.
  16. Mukhopadhyay_CK,_Chatterjee_IB._NADPH-initiated_cytochrome_P450-mediated_free_metal_ion-independent_oxidative_damage_of_microsomal_proteins._Exclusive_prevention_by_ascorbic_acid._J._Biol._Chem._269:_13390-13397,_1994.
 

Number_of_students_awarded/submitted_Ph._D.:_25 Number_of_Ph._D._students_currently_enrolled:_07

 

 

Dr._Rakesh_K._Tyagi,_Professor

 

Prof._Rakesh_K._Tyagi_carried_out_his_doctoral_studies_at_Jawaharlal_Nehru_University,_New_Delhi._Subsequently,_he_pursued_his_research_work_in_the_area_of_‘Molecular_Endocrinology’_as_a_Fienberg_Fellow_at_the_Weizmann_Institute_of_Since,_Israel,_and_later_as_INSERM_international_fellow_in_France._Prior_to_joining_the_‘Special_Centre_for_Molecular_Medicine’_he_was_working_in_a_NIH-sponsored_merit_research_scheme_at_the_University_of_Texas_Health_Science_Centre,_USA. _Currently,_his_laboratory_teaches_and_conducts_research_in_‘Molecular_and_Cellular_Endocrinology’_with_pioneering_contributions_in_understanding_the_functional_and_mechanistic_details_of_nuclear_receptor_actions_in_mammalian_cell_physiology._On_the_administrative_front,_he_served_as_the_Chairperson_of_the_Centre_for_Molecular_Medicine_during_2007-09_and_subsequently_as_the_‘Proctor’_of_Jawaharlal_Nehru_University._In_addition,_he_was_appointed_as_the_‘Director’_at_the_Advanced_Instrumentation_Research_Facility_(AIRF)_at_JNU_during_2015_to_2017._He_is_a_recipient_of_gold_medal_by_the_‘Society_for_Reproductive_Endocrinology_and_Comparative_Endocrinology_for_significant_contribution_in_the_area_of_‘Molecular_and_Cellular_Endocrinology’._During_the_year_2010_was_been_nominated_as_a_Fellow_of_the_National_Academy_of_Sciences,_India._He_is_also_Editor-in-Chief_of_the_‘Journal_of_Endocrinology_and_Reproduction’._Presently,_he_is_serving_as_a_Professor_at_the_Special_Centre_for_Molecular_Medicine._He_has_~80_publications,_one_international_patent_and_two_Indian_patents_to_his_credit.

 

Research_Interests:

 

Molecular_and_Cellular_Endocrinology_with_focus_on_Nuclear_Receptors

The_Nuclear_Receptor_super-family_is_a_large_group_of_ligand-modulated_transcription_factors_with_48_members_presently_identified_in_the_human_genome._Members_of_this_family_of_receptors_are_involved_in_regulation_of_numerous_physiological_and_patho-physiological_processes_and_have_enormous_potential_as_targets_for_the_treatment_of_diseases_such_as_cancer,_diabetes,_coronary_heart_disease_and_asthma._Nuclear_Receptors,_that_include_steroid_hormone_receptors,_are_intra-cellular_transcription_factors_which_regulate_gene_expression_in_response_to_their_cognate_ligands._They_function_either_as_homodimers_or_as_heterodimers_with_retinoid_X_receptor_(RXR)._NRs_are_attractive_targets_for_drug_discovery_because_their_activities_can_be_modulated_by_interacting_ligands_and_have_proved_to_be_‘drug-responsive’._However,_some_newly_discovered_members_of_this_family_of_receptors_remain_incompletely_understood,_both_in_terms_of_physiological_roles_and_activating_ligands._In_brief,_nuclear_receptors_represent_enormous_potential_for_drug_discovery_and_are_continuously_being_examined_to_unravel_the_mysteries_underlying_their_mechanisms_of_actions._Towards_better_understanding_of_the_functional_significance_of_these_transcription_factors_some_of_the_comprehensive_research_projects_are_in_progress_in_our_laboratory._The_role_of_two_key_xenobiotic_receptor_i.e._Pregnane_&_Xenobiotic_Receptor_(PXR),_Constitutive_Androstane_Receptor_(CAR)_in_metabolism_and_clearance_of_endogenous_metabolites_and_xenobiotics_(including_prescription_drugs)_and_the_role_of_androgen/estrogen_receptor_mediated_signaling_in_prostate/breast_cancer_progression_are_under_investigation._In_addition_to_above_research_interests,_mitotic_genome-bookmarking_by_nuclear_receptors_highlighting_a_novel_dimension_in_epigenetic_reprogramming_and_disease_assessment_has_been_part_of_an_emerging_concept_initiated_from_our_laboratory.

 

Ongoing_Projects:

The_laboratory_has_been_supported_by_grants_from_ICMR,_CSIR,_DST,_UGC,_DBT_and_NASF._Currently_ongoing_project: Title:_Investigation_into_polymorphism_and_differential_response_of_Thyroid_Hormone_Receptor_(THR)_by_natural_and_synthetic_small_molecule_modulators._Supported_by_Indian_Council_of_Medical_Research_(ICMR).

 

Collaborations:

BHU,_IIT-Roorkee,_National_Dairy_Research_Institute,_AIIMS-Delhi

 

Selected_Publications

  1. Rizvi_S,_Chhabra_A,_Tripathi_A,_Tyagi_RK_(2023)_Mitotic_genome-bookmarking_by_nuclear_hormone_receptors:_A_novel_dimension_in_epigenetic_reprogramming_and_disease_assessment._Mol_Cell_Endocrinol._18:112069._doi:_10.1016/j.mce.2023.112069_(in_Press)
  2. Kashyap_J,_Kumari_N,_Ponnusamy_K,_Tyagi_RK_(2023)_Hereditary_Vitamin_D-Resistant_Rickets_(HVDRR)_associated_SNP_variants_of_vitamin_D_receptor_exhibit_malfunctioning_at_multiple_levels._BBA-Gene_Regulatory_Mechanisms_Volume_1866:_194891
  3. Agrawal_H,_Thakur_K,_Mitra_S,_Mitra_D,_Keswani_C,_Sircar_D,_Onteru_S,_Singh_D,_Singh_SP,_Tyagi_RK,_Roy_P._(2022)_Evaluation_of_(Anti)androgenic_Activities_of_Environmental_Xenobiotics_in_Milk_Using_a_Human_Liver_Cell_Line_and_Androgen_Receptor-Based_Promoter-Reporter_Assay._ACS_Omega,7:41531-41547
  4. Thakur_K,_Emmagouni_Sharath_Kumar_Goud1,_Jawa_Y,_Keswani_C,_Onteru_S,_Singh_D,_Singh_SP,_Roy_P,_Tyagi_RK_(2023)_Detection_of_endocrine_and_metabolism_disrupting_xenobiotics_in_milk-derived_fat_samples_by_fluorescent_protein-tagged_nuclear_receptors_and_live_cell_imaging._Toxicology_Mechanisms_and_Methods _33:293-306.
  5. Kashyap_J,_Tyagi_RK_(2022)_Mitotic_genome_bookmarking_by_nuclear_receptor_VDR_advocates_transmission_of_cellular_transcriptional_memory_to_progeny_cells_(2022)_Experimental_Cell_Research_417:113193
  6. Saha_P,_Kumar_S,_Datta_K_and_Tyagi_RK_(2021)_Incidence_of_increased_autophagy_in_mifepristone_treated_in_vitro_and_in_vivo_polycystic_ovarian_models_and_its_reversion_upon_thymoquinone_treatment._Journal_of_Steroid_Biochemistry_and_Molecular_Biology._208:105823
  7. Kumar_S,_Vijayan_S,_Dash_AK,_Gourinath_S_and_Tyagi_RK_(2021)_Nuclear_Receptor_SHP_Dampens_Transcription_Function_and_Abrogates_Mitotic_Chromatin_Association_of_PXR_and_ERα_via_Intermolecular_Interactions._BBA-Gene_Regulatory_Mechanisms_1864:194683.
  8. Roy_N,_Verma_D,_Kashyap_J,_Tyagi_RK_and_Prabhakar_A_(2020)_Prototype_of_a_smart_microfluidic_platform_for_the_evaluation_of_SARS-CoV-2_pathogenesis,_along_with_estimation_of_the_effectiveness_of_potential_drug_candidates_and_antigen-antibody_interactions_in_convalescent_plasma_therapy._Transactions_of_the_Indian_National_Academy_of_Engineering _5:_241–250.
  9. Singh_SK,_Yende_AS,_Ponnusamy_K_and_Tyagi_RK_(2019)_A_comprehensive_evaluation_of_anti-diabetic_drugs_on_nuclear_receptor_PXR_platform_Toxicol_In_Vitro_60:_347-358._(Impact_factor_3.07)
  10. Dagar_M,_Singh_JP,_Dagar_G,_Tyagi_RK_and_Bagchi_G_(2019)_Phosphorylation_of_HSP90_by_protein_kinase_A_is_essential_for_the_nuclear_translocation_of_androgen_receptor._J_Biol_Chem_294:8699-8710.
  11. Rana_M,_Dash_AK,_Ponnusamy_K_and_Tyagi_RK_(2018)_Nuclear_localization_signal_region_in_nuclear_receptor_PXR_governs_the_receptor_association_with_mitotic_chromatin._Chromosome_Res._26:_255-276.
  12. Negi_S,_Singh_SK,_Kumar_S,_Kumar_S_and_Tyagi_RK_(2018)_Stable_cellular_models_of_nuclear_receptor_PXR_for_high-throughput_evaluation_of_small_molecules._Toxicol_In_Vitro._52:_222-234.
  13. Dash_AK,_Yende_AS,_Jaiswal_B,_Tyagi_RK_(2017)_Heterodimerization_of_Retinoid_X_Receptor_with_Xenobiotic_Receptor_partners_occurs_in_the_cytoplasmic_compartment:_mechanistic_insights_of_events_in_living_cells._Experimental_Cell_Research_360:337-346.
  14. Dash_AK,_Yende_AS,_Tyagi_RK_(2017)_Novel_application_of_Red_Fluorescent_Protein_(DsRed-Express)_for_the_study_of_functional_dynamics_of_Nuclear_Receptors._Journal_of_Fluorescence_27:_1225–1231.
  15. Kotiya_D,_Jaiswal_B,_Ghose_S,_Kaul_R,_Datta_K_and_Tyagi_RK_(2016)_Role_of_PXR_in_hepatic_cancer:_its_influences_on_liver_detoxification_capacity_and_cancer_progression._PLoS_One_11(10):e0164087.
  16. Rana_M,_Devi_S,_Gourinath_S,_Goswami_R,_Tyagi_RK_(2016)_A_comprehensive_analysis_and_functional_characterization_of_naturally_occurring_non-synonymous_variants_of_nuclear_receptor_PXR._Biochimica_et_Biophysica_Acta_–_Gene_Regulatory_Mechanisms_1859:_1183–1197.
  17. Priyanka,_Kotiya_D,_Rana_M,_Subbarao_N,_Puri_N_and_Tyagi_RK_(2016)_Transcription_regulation_of_nuclear_receptor_PXR:_role_of_SUMO-1_modification_and_NDSM_in_receptor_function._Molecular_and_Cellular_Endocrinology_420:194-207.
  18. Saradhi_M,_Kumari_S,_Rana_M,_Mukhopadhyay_G_and_Tyagi_RK_(2015)_Identification_and_interplay_of_sequence_specific_DNA_binding_proteins_involved_in_regulation_of_human_Pregnane_&_Xenobiotic_Receptor_Gene_Experimental_Cell_Research_339:187-196.
  19. Kumari_S,_Saradhi_M,_Rana_M,_Chatterjee_S,_Aumercier_M,_Mukhopadhyay_G_and_Tyagi_RK_(2015)_Pregnane_&_Xenobiotic_Receptor_gene_expression_in_liver_cells_is_modulated_by_Ets-1_in_synchrony_with_transcription_factors_Pax5,_LEF-1_and_c-Jun._Experimental_Cell_Research_330:_398-411.
  20. Kumar_S_and_Tyagi_RK_(2012)_Androgen_receptor_association_with_mitotic_chromatin_-_analysis_with_introduced_deletions_and_disease-inflicting_mutations._FEBS_Journal_279:4598-4614.(journal_cover_article)
 

Professor_Suman_Kumar_Dhar

 

Prof._Suman_Kumar_Dhar_carried_out_his_doctoral_studies_(1992-98)_from_the_School_of_Environmental_Sciences,_Jawaharlal_Nehru_University,_New_Delhi_working_on_replication_and_maintenance_of_ribosomal_DNA_circle_in_the_protozoan_parasite_Entamoeba_histolytica._Prior_to_joining_the_Special_Centre_for_Molecular_Medicine,_he_worked_on_mammalian_DNA_replication_as_post-doctoral_fellow_(1998-2001)_at_the_Brigham_and_Women’s_Hospital,_Harvard_Medical_School,_Boston,_USA._

 

Research_Interests:

 

Prof._Suman_Kumar_Dhar_at_the_Special_Centre_for_Molecular_Medicine,_JNU_is_studying_replication_and_cell_cycle_control_of_two_medically_important_human_pathogens:_Plasmodium_falciparum_that_causes_human_malaria_and_Helicobacter_pylori_that_causes_gastric_ulcer_and_gastric_adenocarcinoma._Both_these_pathogens_are_immensely_important_but_their_biology_is_poorly_understood._Disease_control_is_hampered_due_to_drug_resistance_and_non-availability_of_vaccine._His_main_aim_is_to_understand_the_complex_DNA_replication_and_cell_cycle_mechanisms_in_these_pathogens_with_a_view_to_identify_novel_targets_for_therapy.

His_laboratory_discovered_a_mechanism_by_which_the_unique_DnaB_helicase_ring_is_loaded_in_H._pylori_oriC_without_the_use_of_DnaC,_conventional_helicase_loader,_when_he_identified_Hp0897,_an_unknown_ORF_as_the_helicase_loader_in_H._pylori._Further_research_from_his_laboratories_has_shown_unique_polar_replisome_and_divisome_assembly_in_H._pylori_that_leads_to_asymmetric_growth_of_these_pathogenic_bacteria._

Multiple_rounds_of_DNA_replication_take_place_during_the_blood_stage_developmental_cycle_of_P._falciparum_where_1N_DNA_content_goes_up_to_16-32_N._Dr_Dhar_is_studying_the_control_of_both_organellar_apicoplast_DNA_and_nuclear_DNA_replication._He_has_functionally_characterized_key_molecules_that_are_essential_for_nuclear/organellar_DNA_replication._He_has_also_discovered_the_presence_of_ARS-like_DNA_sequences_in_the_Plasmodium_genome_and_identified_genome-wide_distribution_of_replication_initiation_sites_in_these_parasites._The_presence_of_high_frequency_replication_initiation_sites_in_Plasmodium_genome_may_explain_the_faster_cycles_of_DNA_replication_in_this_deadly_pathogen._

Using_Chemical_Biology_approach,_his_laboratory_has_identified_potent_inhibitors_against_H._pylori_and_P._falciparum_that_has_led_to_US_and_Indian_patents.

 

Ongoing_Projects:_

    Deciphering_functional_role_of_human_pathogenic_bacteria_Helicobacter_pylori_arginine_decarboxylase;_funded_by_Indian_Council_of_Medical_Research,_India_(2023-26).
The_above_proposal_aims_to_understand_the_role_of_H._pylori_arginine_decarboxylase_in_acid_response/adaptation_and_DNA_replication_leading_to_the_possibility_of_using_it_as_a_therapeutic_target_against_H._pylori

Studies_on_replication_and_cell_division_of_two_medically_important_human_pathogens_Helicobacter_pylori_and_Plasmodium_falciparum:_unique_biology_and_finding_targets_for_intervention;_funded_by_Sir_J._C._Bose_National_Fellowship,_SERB,_DST,_Govt_of_India_(2021-26). Studying_the_H._pylori_replisome_and_cell_divisome_and_their_coordination_in_the_context_of_mammalian_host_cells_will_help_to_shed_some_insight_into_the_interaction_between_the_host_and_pathogen._These_insights_will_have_possible_applications_in_managing_H._pylori_infections_as_inhibition_of_DNA_replication_and_cell_division_indeed_will_have_effect_on_bacterial_growth_and_multiplication._Studying_functional_aspects_of_selected_Plasmodium_falciparum_parasite_proteins_like_PfORC,_PfMCM,_PfPCNA,_PfGCN5_etc_will_not_only_help_to_understand_the_unique_aspects_of_parasite_biology_ranging_from_parasite_DNA_replication,_intracellular_trafficking_to_gene_regulation,_it_will_also_help_to_establish_new_target_for_therapy.

 

Collaborations:

 

Dr_A._Krishnamachari,_Prof._Naidu_Subbarao,_SCIS,_JNU;_Dr_Neelima_Mondal,_SLS,_JNU;_Prof._Pritam_Mukhopadhyay,_SPS,_JNU,_Dr_Inderjeet_Kaur,_Central_University_of_Haryana

Dr_Asish_Mukhopadhyay,_NICED,_Kolkata;_Dr_Agam_P._Singh,_NII,_New_Delhi_

 

Selected_Publications:

 

>80_publications,_H-index:_27;_Total_citations_>3700_(Google_scholar)

Papers_published_from_JNU_as_independent_researcher

Research_articles:

  1. Shekhar_S,_Verma_S,_Gupta_MK,_Roy_SS,_Kaur_I,_Krishnamachari_A,_Dhar_SK._(2023)_Genome-wide_binding_sites_of_Plasmodium_falciparum_mini_chromosome_maintenance_protein_MCM6_show_new_insights_into_parasite_DNA_replication._Biochim_Biophys_Acta_Mol_Cell_Res._1870:119546._doi:_10.1016/j.bbamcr.2023.119546._(IF:_5.0)
  2. Shekhar_S,_Bhowmick_K,_Dhar_SK._(2022)_Role_of_PfMYST_in_DNA_replication_in_Plasmodium_falciparum. _Exp_Parasitol._242:108396._(IF:_2.2)
  3. Tehlan_A,_Bhowmick_K,_Kumar_A,_Subbarao_N,_Dhar_SK._(2022)_The_tetrameric_structure_of_Plasmodium_falciparum_phosphoglycerate_mutase_is_critical_for_optimal_enzymatic_activity._J_Biol_Chem._298:101713._(IF:_5.1)
  4. Purushothaman_M,_Dhar_SK,_Natesh_R._(2022)_Role_of_unique_loops_in_oligomerization_and_ATPase_function_of_Plasmodium_falciparum_gyrase_B._Protein_Sci._31:323-332._(IF:_6.1)
  5. Valissery_P, _Thapa_R, _Singh_J,_Gaur_D,_Bhattacharya_J,_Singh_AP_and_Dhar_SK._(2020)_Potent_in_vivo_antimalarial_activity_of_water-soluble_artemisinin_nano-preparations._RSC_Advance._10:_36201–36211._(IF:_3.2)
  6. Tehlan_A,_Karmakar_BC,_Paul_S,_Kumar_R,_Kaur_I,_Ghosh_A,_Mukhopadhyay_AK,_Dhar_SK._(2020)_Antibacterial_action_of_acriflavine_hydrochloride_for_eradication_of_the_gastric_pathogen_Helicobacter_pylori._FEMS_Microbiol_Lett._367:_1-9._(IF:_2.7)
  7. Dana_S,_Valissery_P,_Kumar_S,_Gurung_SK,_Mondal_N,_Dhar_SK*,_Mukhopadhyay_P*._(*co-corresponding_author)_(2020)_Synthesis_of_Novel_Ciprofloxacin-Based_Hybrid_Molecules_toward_Potent_Antimalarial_Activity._ACS_Med_Chem_Lett._11:1450-1456._(IF:_4.3)
  8. Bhowmick_K,_Tehlan_A,_Sunita,_Sudhakar_R,_Kaur_I,_Sijwali_PS,_Krishnamachari_A,_Dhar_SK._(2020)_Plasmodium_falciparum_GCN5_acetyltransferase_follows_a_novel_proteolytic_processing_pathway_that_is_essential_for_its_function._J_Cell_Sci._133(1):jcs236489._(IF:_4.1)
  9. Kumar_A,_Saha_A,_Verma_VK,_Dhar_SK._(2019)_Helicobacter_pylori_helicase_loader_protein_Hp0897_shows_unique_functions_of_N-_and_C-terminal_regions._Biochem_J._476:3261-3279._(IF:_3.8)
  10. Pradhan_S,_Kalia_I,_Roy_SS,_Singh_OP,_Adak_T,_Singh_AP,_Dhar_SK._(2019)_Molecular_characterization_and_expression_profile_of_an_alternate_proliferating_cell_nuclear_antigen_homolog_PbPCNA2_in_Plasmodium_berghei._IUBMB_Life._71:1293-1301._(IF:_3.5)_ 
  11. Banu_K,_Mitra_P,_Subbarao_N,_Dhar_SK._(2018)_Role_of_tyrosine_residue_(Y213)_in_nuclear_retention_of_PCNA1_in_human_malaria_parasite_Plasmodium_falciparum._FEMS_Microbiol_Lett._365(17)._(IF:_2.7)
  12. Kamran_M,_Dubey_P,_Verma_V,_Dasgupta_S,_Dhar_SK._(2018)_Helicobacter_pylori_shows_asymmetric_and_polar_cell_divisome_assembly_associated_with_DNA_replisome._FEBS_J._285:2531-2547._(IF:_5.5)
  13. Kumar_A,_Dhar_SK,_Subbarao_N._(2018)_In_silico_identification_of_inhibitors_against_Plasmodium_falciparum_histone_deacetylase_1_(PfHDAC-1)_J_Mol_Model._24:_232._(IF:_1.7)
  14. Sharma_R,_Sharma_B,_Gupta_A,_Dhar_SK._(2018)_Identification_of_a_novel_trafficking_pathway_exporting_a_replication_protein,_Orc2_to_nucleus_via_classical_secretory_pathway_in_Plasmodium_falciparum._Biochim_Biophys_Acta_Mol_Cell_Res._1865:817-829._(IF:_4.1)
  15. Gupta_MK,_Agarawal_M,_Banu_K,_Reddy_KS,_Gaur_D,_Dhar_SK._(2018)_Role_of_Chromatin_assembly_factor_1_in_DNA_replication_of_Plasmodium_falciparum._Biochem_Biophys_Res_Commun._495:1285-1291._(IF:_3.3)
  16. Kumar_A,_Bhowmick_K,_Vikramdeo_KS,_Mondal_N,_Subbarao_N,_Dhar_SK._(2017)_Designing_novel_inhibitors_against_histone_acetyltransferase_(HAT:_GCN5)_of_Plasmodium_falciparum._Eur_J_Med_Chem._138:26-37._(IF:_6.1)
  17. Agarwal_M,_Bhowmick_K,_Shah_K,_Krishnamachari_A,_Dhar_SK._(2017)_Identification_and_characterization_of_ARS-like_sequences_as_putative_origin(s)_of_replication_in_human_malaria_parasite_Plasmodium_falciparum._FEBS_J._284:2674-2695._(IF:_5.5)
  18. Dana_S,_Keshri_SK,_Shukla_J,_Vikramdeo_KS,_Mondal_N,_Mukhopadhyay_P,_Dhar_SK._(2016)_Design,_Synthesis_and_Evaluation_of_Bifunctional_Acridinine-Naphthalenediimide_Redox-Active_Conjugates_as_Antimalarials._ACS_Omega._1:318-333._(IF:_3.3)
  19. Kamran_M,_Sinha_S,_Dubey_P,_Lynn_AM,_Dhar_SK._(2016)_Identification_of_putative_Z-ring-associated_proteins,_involved_in_cell_division_in_human_pathogenic_bacteria_Helicobacter_pylori._FEBS_Lett._590:2158-71._(IF:_3.5)
  20. Verma_V,_Kumar_A,_Nitharwal_RG,_Alam_J,_Mukhopadhyay_AK,_Dasgupta_S,_Dhar_SK. _(2016)_Modulation_of_the_enzymatic_activities_of_replicative_helicase_(DnaB)_by_interaction_with_Hp0897:_a_possible_mechanism_for_helicase_loading_in_Helicobacter_pylori._Nucleic_Acids_Res._44:3288-303_(IF:_19.1)
  21. Deshmukh_AS,_Agarwal_M,_Dhar_SK._(2016)_Regulation_of_DNA_replication_proteins_in_parasitic_protozoans:_possible_role_of_CDK-like_kinases._Curr_Genet._62:481-6_(IF:_2.6)
  22. Narayanaswamy_N,_Das_S,_Samanta_PK,_Banu_K,_Sharma_GP,_Mondal_N,_Dhar_SK,_Pati_SK,_Govindaraju_T._(2015)_Sequence-specific_recognition_of_DNA_minor_groove_by_an_NIR-fluorescence_switch-on_probe_and_its_potential_applications._Nucleic_Acids_Res._43:8651-63_(IF:_19.1)
  23. Mitra_P,_Banu_K,_Deshmukh_AS,_Subbarao_N,_Dhar_SK._(2015)_Functional_dissection_of_proliferating-cell_nuclear_antigens_(1_and_2)_in_human_malarial_parasite_Plasmodium_falciparum:_possible_involvement_in_DNA_replication_and_DNA_damage_response._Biochem_J._470:115-29._(IF:_3.8)
  24. Deshmukh_AS,_Agarwal_M,_Mehra_P,_Gupta_A,_Gupta_N,_Doerig_CD,_Dhar_SK._(2015)_Regulation_of_Plasmodium_falciparum_Origin_Recognition_Complex_subunit_1_(PfORC1)_function_through_phosphorylation_mediated_by_CDK-like_kinase_PK5._Mol_Microbiol._98:17-33._(IF_3.5)
  25. Narayanaswamy_N,_Kumar_M,_Das_S,_Sharma_R,_Samanta_PK,_Pati_SK,_Dhar_SK,_Kundu_TK,_Govindaraju_T_(2014)._A_Thiazole_Coumarin_(TC)_Turn-On_Fluorescence_Probe_for_AT-Base_Pair_Detection_and_Multipurpose_Applications_in_Different_Biological_Systems._Sci_Rep._4:6476._(IF_4.1)
  26. Srivastava_S,_Bhowmick_K,_Chatterjee_S,_Basha_J,_Kundu_TK,_Dhar_SK._(2014)_Histone_H3K9_acetylation_level_modulates_gene_expression_and_may_affect_parasite_growth_in_human_malaria_parasite_Plasmodium_falciparum._FEBS_J._281:5265-78_(IF_5.5)
  27. Dana_S,_Prusty_D,_Dhayal_D,_Gupta_MK,_Dar_A,_Sen_S,_Mukhopadhyay_P,_Adak_T,_Dhar_SK._(2014)_The_potent_Anti-malarial_activity_of_Acriflavine_in_vitro_and_in_vivo._ACS_Chem_Biol._9:2366-73._(IF_4.5)
  28. Sharma_A,_Kamran_M,_Verma_V,_Dasgupta_S,_Dhar_SK._(2014)_Intracellular_Locations_of_Replication_Proteins_and_the_Origin_of_Replication_during_Chromosome_Duplication_in_the_Slowly_Growing_Human_Pathogen_Helicobacter_pylori._J_Bacteriol._196:999-1011._Journal_cover_article._(IF_2.9)
  29. Bhowmick_K,_Dhar_SK._(2013)_Plasmodium_falciparum_single-stranded_DNA-binding_protein_(PfSSB)_interacts_with_PfPrex_helicase_and_modulates_its_activity._FEMS_Microbiol_Lett._2013_Nov_23._doi:_10.1111/1574-6968._(IF_2.7)
  30. Abdul_Rehman_SA,_Verma_V,_Mazumder_M,_Dhar_SK1,_Gourinath_S1._(2013)_Crystal_structure_and_mode_of_helicase_binding_of_the_C-terminal_domain_of_primase_from_Helicobacter_pylori._J_Bacteriol._195:2826-38._(1Co-corresponding_author)_(IF_2.9)
  31. Deshmukh_A,_Srivastava_S,_Herrmann_S,_Gupta_A,_Mitra_P,_Gilberger_TW_and_Dhar_SK._(2012)_The_role_of_N-terminus_of_Plasmodium_falciparum_ORC1_in_telomeric_localization_and_var_gene_silencing._Nucleic_Acids_Research_40:5313-31_(IF_19.1)
  32. Nitharwal_RG,_Verma_V,_Subbarao_N,_Dasgupta_S,_Choudhury_NR_and_Dhar_SK._(2012)_DNA_binding_activity_of_Helicobacter_pylori_DnaB_helicase:_the_role_of_the_N-terminal_domain_in_modulating_DNA_binding_activities._FEBS_J._279:234-50._Journal_cover_article._(IF._5.5)
  33. Dorin-Semblat_D,_Carvalho_TG,_Nivez_M,_Halbert_J,_…Mehra_P,_Dhar_S,…Tilley_L,_Doerig_C._(2012)_An_atypical_cyclin-dependent_kinase_controls_Plasmodium_falciparum_proliferation_rate._Kinome._1:_4-16.
  34. Prusty_D,_Dar_A,_Priya_R,_Sharma_A,_Dana_S,_Choudhury_NR,_Rao_NS_and_Dhar_SK._(2010)._Single-stranded_DNA_binding_protein_from_human_malarial_parasite_Plasmodium_falciparum_is_encoded_in_the_nucleus_and_targeted_to_the_apicoplast._Nucleic_Acids_Res._38:7037-53_(IF_19.1)
  35. Kashav_T,_Nitharwal_R,_Abdulrehman_SA,_Gabdoulkhakov_A,_Saenger_W,_Dhar_SK*,_Gourinath_S*._(2009)_Three-dimensional_structure_of_N-terminal_domain_of_DnaB_helicase_and_helicase-primase_interactions_in_Helicobacter_pylori._PLoS_One._20:_e7515._(*_co-corresponding_author)_(IF_3.5)
  36.  Dar_A,_Prusty_D,_Mondal_N_and_Dhar_SK._(2009)_A_Unique_45_Amino  _Acid_Region_in_the_Toprim_Domain_of_Plasmodium_falciparum_Gyrase_B_is_Essential_for_Its_Activity._Eukaryot_Cell_8:_1759-69._(IF_3.0)
  37. Gupta_A,_Mehra_P,_Deshmukh_A,_Dar_A,_Mitra_P,_Roy_N_and_Dhar_SK._(2009) _Functional_dissection_of_the_catalytic_carboxyl-terminal_domain_of_human_malaria_parasite_Plasmodium_falciparum_origin_recognition_complex_subunit_1_(PfORC1)._Eukaryot_Cell_8:_1341-51(IF_3.0)
  38. Sharma_A.,_Nitharwal_RG.,_Singh_B.,_Dar_A.,_Dasgupta_A_and_Dhar_SK._(2009)_Helicobacter_pylori_single-stranded_DNA_binding_protein-functional_characterization_and_modulation_of_H._pylori_DnaB_helicase_activity._FEBS_J._276:_519-531_(IF_5.5)
  39. Gupta,_A.,_Mehra_P._and_Dhar_SK._(2008)._Plasmodium_falciparum_origin_recognition_complex_subunit_5:_functional_characterization_and_role_in_DNA_replication_foci_formation._Molecular_Microbiology_69:_646-65._(IF_3.5)
  40. Prusty_D.,_Mehra_P.,_Srivastava_S.,_Shivange_AV.,_Gupta_A.,_Roy_N_and_Dhar_SK._(2008)_Nicotinamide_inhibits_Plasmodium_falciparum_Sir2_activity_in_vitro_and_parasite_growth._FEMS_Microbiol_Lett._282:_266-72._(IF_2.7)
  41. Nitharwal_RG,_Paul_S,_Soni_RK,_Sinha_S,_Prusthy_D,_Keshav_T,_RoyChoudhury_N,_Mukhopadhyay_G,_Chaudhury_T,_Gourinath_S,_and_Dhar_SK._(2007)_The_domain_structure_of_Helicobacter_pylori_DnaB_helicase:_The_N-terminal_domain_can_be_dispensable_for_helicase_activity_whereas_the_extreme_C-terminal_region_is_essential_for_its_function._Nucleic_Acids_Res._35:_2861-74_(IF_19.1)
  42. Dar_MA,_Sharma_A,_Mondal_N_and_Dhar_SK._(2007)_Molecular_cloning_of_apicoplast_targeted_Plasmodium_falciparum_DNA_gyrase_genes:_unique_intrinsic_ATPase_activity_and_ATP-independent_dimerisation_of_PfGyrB_subunit._Eukaryotic_Cell._6:398-412._(IF_3.0)
  43. Gupta_A,_Mehra_P,_Nitharwal_R,_Sharma_A,_Biswas_AK_and_Dhar_SK._(2006)_Analogous_expression_pattern_of_Plasmodium_falciparum_replication_initiation_proteins_PfMCM4_and_PfORC1_during_the_asexual_and_sexual_stages_of_intraerythrocytic_developmental_cycle._FEMS_Microbiol._Lett._261:12-8._(IF_2.7)
  44. Mehra_P,_Biswas_AK,_Gupta_A,_Gourinath_S,_Chitnis_CE_and_Dhar_SK._(2005)_Expression_and_characterization_of_human_malaria_parasite_Plasmodium_falciparum_origin_recognition_complex_subunit_1._Biochem_Biophys_Res_Commun._337:955-66._(IF_3.3)
  45. Soni_RK,_Mehra_P,_Mukhopadhyay_G_and_Dhar_SK._(2005)_Helicobacter_pylori_DnaB_helicase_can_bypass_E._coli_DnaC_function_in_vivo._Biochem_J._389(Pt_2):541-8_(IF_4.4)
  46. Soni_RK,_Mehra_P,_Choudhury_NR,_Mukhopadhyay_G,_Dhar_SK._(2003)_Functional_characterization_of_Helicobacter_pylori_DnaB_helicase._Nucleic_Acids_Res._31:6828-40._(IF_19.1)
  47. Jha_S,_Karnani_N,_Dhar_SK,_Mukhopadhayay_K,_Shukla_S,_Saini_P,_Mukhopadhayay_G,_Prasad_R._(2003)._Purification_and_characterization_of_the_N-terminal_nucleotide_binding_domain_of_an_ABC_drug_transporter_of_Candida_albicans:_uncommon_cysteine_193_of_Walker_A_is_critical_for_ATP_hydrolysis._Biochemistry_42:10822-32._(IF_3.0)  _ 
  48. Dhar,_S.K$.,_Mondal,_N.,_Soni,_R.K.,_and_Mukhopaddhyay,_G._(2002)._A_~35_kDa_polypeptide_from_baculovirus_infected_insect_cells_binds_to_yeast_ACS_like_elements_in_the_presence_of_ATP._BMC_Biochemistry_2002,_3:23_($corresponding_author)_(IF_2.5)

 

 Scientific_reviews

  1. Tehlan_A,_Saha_A_and_Dhar_SK_(2023)_Targeting_proteases_and_proteolytic_processing_of_unusual_N-terminal_extensions_of_Plasmodium_proteins:_parasite_peculiarity._Frontiers_in_Drug_Discovery._3:1223140._doi:_10.3389/fddsv.2023.1223140
  2. Deshmukh_AS,_Srivastava_S,_Dhar_SK._(2013)_Plasmodium_falciparum:_epigenetic_control_of_var_gene_regulation_and_disease._Subcell_Biochem._61:659-82.
  3. Mitra_P,_Deshmukh_A_and_Dhar_SK._(2012)_DNA_replication_during_intra-erythrocytic_stages_of_human_malarial_parasite_Plasmodium_falciparum._Current_Science._102;725-740.
  4. Nitharwal_RG,_Verma_V,_Dasgupta_S,_Dhar_SK._(2011)_Helicobacter_pylori_chromosomal_DNA_replication:_current_status_and_future_perspectives._FEBS_Lett._585:7-17.
  5. Dhar,_S._K$.,_Soni_R._K.,_Das,_B._K._and_Mukhopaddhyay,_G._(2003)_Molecular_Mechanism_of_Action_of_Major_Helicobacter_pylori_Virulence_Factors._Molecular_and_Cellular_Biochemistry_253:207-15_($corresponding_author)

 

Number_of_students_awarded/submitted_Ph._D.:_28 Number_of_Ph._D._students_currently_enrolled:_8

 

Professor_Anand_Ranganathan

Prof._Anand_Ranganathan_obtained_his_BSc_(Hons)_degree_in_Chemistry_from_St._Stephen’s_College,_Delhi,_after_which_he_went_on_a_scholarship_to_Cambridge,_UK,_where_he_obtained_his_BA_(Tripos)_in_Natural_Sciences,_his_MA,_and_his_PhD._After_a_post-doctoral_stint_at_Cambridge,_Anand_returned_to_India_to_join_International_Centre_for_Genetic_Engineering_and_Biotechnology,_Delhi,_where_he_ran_his_lab_for_16_years_as_a_Staff_Research_Scientist._In_2015_he_joined_the_Special_Centre_for_Molecular_Medicine,_Jawaharlal_Nehru_University,_Delhi_and_became_a_full_Professor_in_2019._His_laboratory_works_in_the_area_of_Directed_Evolution_and_Pathogenesis,_with_special_emphasis_on_Tuberculosis_and_Malaria._Scientific_contributions_from_Anand's_lab_have_been_published_in_peer-reviewed_journals_like_The_Journal_of_Biological_Chemistry,_Chemistry_&_Biology,_The_Journal_of_Infectious_Diseases,_Journal_of_Clinical_Investigation,_and_Nature_Communications.

 

Books_(non-scientific):

  1. Fiction:_The_Land_of_the_Wilted_Rose_(Rupa,_2012)
  2. Fiction:_For_Love_and_Honour_(Bloomsbury,_2015)
  3. Fiction:_The_Rat_Eater_(Bloomsbury,_2019)
  4. Fiction:_Souffle_(Penguin,_2023).
  5. Non-fiction:_Hindus_in_Hindu_Rashtra_(BluOne_Ink,_2023).
  6. Non-fiction:_India’s_Forgotten_Scientists_(Penguin,_2024_slated).

 

 

Research_Interests:

Prof._Ranganathan’s_Laboratory_has_invented_codon-shuffling,_a_novel_method_for_the_directed_evolution_of_proteins,_using_which_a_de_novo_protein_was_unearthed_that_was_able_to_disrupt_ICAM_dimerisation_and_block_host_cell_invasion_by_both_Mycobacterium_tuberculosis_and_Plasmodium_falciparum._The_major_objectives_of_this_research_group_are_as_follows:_1._To_study_the_role_of_host_ICAMs_in_cell_invasion_by_Mycobacterium_tuberculosis_and_Plasmodium_falciparum._2._To_discover_new_molecules_for_TB_therapy._3._To_focus_on_the_emergence_of_Plasmodium_falciparum_resistance_and_the_repurposing_of_existing_drugs_against_artemisinin-resistant_Plasmodium_falciparum._4._To_discover_de-novo_peptide_binders_against_target_proteins_of_Mycobacterium_tuberculosis,_Plasmodium_falciparum,_and_Leishmania_donovani.

 

Ongoing_Projects:

Title:_A_multi-targeted_approach_encompassing_fundamental_and_applied_studies_towards_drug_discovery_for_Leishmaniasis

Abstract: Leishmaniasis_is_one_of_the_major_neglected_tropical_diseases,_for_which_no_vaccines_exist._Chemotherapy_is_hampered_by_limited_efficacy_coupled_with_development_of_resistance_and_other_side_effects._The_Leishmania-macrophage_interaction_provides_an_excellent_example_of_co-evolution_that_promotes_parasite_survival_and_causes_diseases._Conceivably,_interfering_with_these_processes_represents_a_promising_new_strategy_against_Leishmania._For_this,_a_multi_targeted_approach_is_required_to_get_better_understanding_of_these_host_pathogen_interactions_to_develop_a_drug._Thus,_we_proposed_to_target_four_essential_mechanisms_of_its_pathogenesis_to_block_motility,_invasion,_growth,_sustenance_inside_the_host_macrophages.

Name_of_the_funding_agency:_DST-IRHPA_grant_number_IPA/2020/000007,_Duration_(From-To)_27.03.2020-27.03.2025

 

Collaborations:

SCMM,_JNU;_THSTI,_Faridabad;_IIT,_Delhi.

 

Selected_Publications:

  1. Chaurasiya,_A.,_Kumari,_G.,_Garg,_S.,_Shoaib,_R.,_Anam,_Z.,_Joshi,_N.,_Kumari,_J.,_Singhal,_J.,_Singh,_N.,_Kaushik,_S.,_Kahlon,_AK.,_Dubey,_N.,_Maurya,_MK.,_Srivastava,_P.,_Marothia,_M.,_Joshi,_P.,_Gupta,_K.,_Saini,_S.,_Das,_G.,_Bhattacharjee,_S.,_Singh,_S*.,_Ranganathan,_A*._2022._Targeting_Artemisinin-Resistant_Malaria_by_Repurposing_the_Anti-Hepatitis_C_Virus_Drug_Alisporivir._Antimicrob_Agents_Chemother._66(12):_e0039222.
  2. Anam,_Z.,_Kumari,_G.,_Mukherjee,_S.,_Rex,_DAB.,_Biswas,_S.,_Maurya,_P.,_Ravikumar,_S.,_Gupta,_N.,_Kushawaha,_AK.,_Sah,_RK.,_Chaurasiya,_A.,_Singhal,_J.,_Singh,_N.,_Kaushik,_S.,_Prasad,_TSK.,_Pati,_S.,_Ranganathan,_A.*,_Singh,_S*._2022._Complementary_crosstalk_between_palmitoylation_and_phosphorylation_events_in_MTIP_regulates_its_role_during_Plasmodium_falciparum_invasion._Front_Cell_Infect_Microbiol._12:924424.
  3. Singhal,_J*.,_Madan,_E.,_Chaurasiya,_A.,_Srivastava,_P.,_Singh,_N.,_Kaushik,_S.,_Kahlon,_AK.,_Maurya,_MK.,_Marothia,_M.,_Joshi,_P.,_Ranganathan,_A*._and_Singh,_S*._2022._Host_SUMOylation_Pathway_Negatively_Regulates_Protective_Immune_Responses_and_Promotes_Leishmania_donovani_Survival._Front._Cell._Infect._Microbiol._12:878136.
  4. Srivastava,_A.,_Garg,_S.,_Karan,_S.,_Kaushik,_S.,_Ranganathan,_A.,_Pati,_S.,_Garg,_LC,_Singh,_S._2022._Plasmodium_falciparum_Antigen_Expression_in_Leishmania_Parasite:_A_Way_Forward_for_Live_Attenuated_Vaccine_Development._Methods_Mol_Biol._2410:555.
  5. Singh,_DK.,_Tousif,_S.,_Bhaskar,_A.,_Devi,_A.,_Negi,_K.,_Moitra,_B.,_Ranganathan,_A.,_Dwivedi,_VP.,_Das,_G._2021._Luteolin_as_a_potential_host-directed_immunotherapy_adjunct_to_isoniazid_treatment_of_tuberculosis._PLoS_Pathog._17:8.
  6. Chaurasiya,_A.,_Garg,_S.,_Khanna,_A.,_Narayana,_C.,_Dwivedi,_VP.,_Joshi,_N.,_Anam,_ZE.,_Singh,_N.,_Singhal,_J.,_Kaushik,_S.,_Kahlon,_AK,_Srivastava,_P.,_Marothia,_M.,_Kumar,_M.,_Kumar,_S.,_Kumari,_G.,_Munjal,_A.,_Gupta,_S.,_Singh,_P.,_Pati,_S.,_Dag,_G.,_Sagar,_R.,_Ranganathan,_A*.,_Singh,_S*._2021._Pathogen_induced_subversion_of_NAD+_metabolism_mediating_host_cell_death:_a_target_for_development_of_chemotherapeutics._Cell_Death_Discov._7:10.
  7. Anam,_ZE.,_Joshi,_N.,_Gupta,_S.,_Yadav,_P.,_Chaurasiya,_A.,_Kahlon,_AK.,_Kaushik,_S.,_Munde,_M.,_Ranganathan,_A*.,_Singh,_S*._2020._A_De_novo_Peptide_from_a_High_Throughput_Peptide_Library_Blocks_Myosin_A_-MTIP_Complex_Formation_in_Plasmodium_falciparum._Int._J._Mol._Sci._21(17):6158.
  8. Singh,_DK.,_Dwivedi,_VP.,_Singh,_SP.,_Kumari,_A.,_Sharma,_SK.,_Ranganathan,_A.,_Van_Kaer,_L.,_Das,_G._2020._Luteolin-mediated_Kv1.3_K+_channel_inhibition_augments_BCG_vaccine_efficacy_against_tuberculosis_by_promoting_central_memory_T_cell_responses_in_mice._PLoS_Pathog._16:9.
  9. Prakash,_P.,_Zeeshan,_M.,_Saini,_E.,_Muneer,_A.,_Khurana,_S.,_Chourasia,_BK.,_Deshmukh,_A.,_Kaur,_I.,_Dabra,_S.,_Singh,_N.,_Anam,_Z.,_Chaurasiya,_A.,_Kaushik,_S.,_Dahiya,_P.,_Kalamuddin,_M.,_Thakur,_JK.,_Mohmmed,_A.,_Ranganathan,_A.*,_Malhotra,_P*._2017._Human_Cyclophilin_B_forms_part_of_a_multi-protein_complex_during_erythrocyte_invasion_by_Plasmodium_falciparum._Nat_Commun._8(1):1548.
  10. Bhalla,_K.,_Chugh,_M.,_Mehrotra,_S.,_Rathore,_S.,_Tousif,_S.,_Dwivedi,_VP.,_Prakash,_P.,_Samuchiwal,_SK.,_Kumar,_S.,_Singh,_DK.,_Ghanwat,_S.,_Kumar,_D.,_Das,_G.,_Mohmmed,_A.,_Malhotra,_P.*,_Ranganathan_A*._2015._Host_ICAMs_play_a_role_in_cell_invasion_by_Mycobacterium_tuberculosis_and_Plasmodium_falciparum._Nat_Commun._6:6049.

 

Number_of_students_awarded/submitted_PhD:_13

Number_of_PhD_students_currently_enrolled:_06

 

 

Umesh_Chand_Singh_Yadav,_Professor

 

Prof._Umesh_Chand_Singh_Yadav_is_presently_affiliated_with_the_Special_Centre_for_Molecular_Medicine,_JNU,_New_Delhi_as_a_Professor._He_has_earned_a_Ph.D._degree_in_Biochemistry_from_School_of_Life_Sciences,_JNU._After_completing_his_Ph.D.,_he_worked_as_a_postdoctoral_fellow_for_6_years_at_the_University_of_Texas_Medical_Branch_Galveston,_USA_and_was_promoted_to_the_faculty_position_and_worked_for_3_years_as_faculty_at_the_University_of_Texas_Medical_Branch_Galveston,_USA._Has_was_awarded_Eye_Research/Retina_Research_Foundation/_Joseph_M._and_Eula_C._Lawrence_Scholarship_Travel_Grant_by_ARVO_Foundation_in_2007._

 

He_returned_to_India_in_2013_with_an_award_of_Ramanujan_Fellowship_from_Department_of_Science_and_Technology_(DST),_Govt_of_India_and_joined_as_Assistant_Professor_at_the_Central_University_of_Gujarat,_Gandhinagar_where_he_ascended_to_the_Professor_position_in_2020._Subsequently_he_moved_to_Jawaharlal_Nehru_University_(JNU)_and_joined_Special_Centre_for_Molecular_Medicine_(SCMM)_as_a_Professor._

 

His_area_of_research_is_Metabolic_Disorder_&_Inflammatory_Diseases_including_Cancer,_diabetes,_cardiovascular_diseases_and_lung_inflammatory_diseases_like_asthma_and_COPD._He_has_published_more_than_85_manuscripts,_review_articles_and_book_chapters_and_edited_two_books_for_Springer_Nature_on_the_topics_of_“Oxidative_stress_and_human_diseases”_and_“Functional_food”._He_has_successfully_completed_4_extramurally_funded_projects_awarded_by_DST/SERB,_DBT,_and_GSBTM_and_executing_many_other_extramurally_funded_projects_at_SCMM,_JNU_in_niche_areas_of_his_expertise._He_also_holds_an_international_patent_on_COPD_and_another_Indian_patent_is_filed_on_the_treatment_modalities_of_Endothelial_dysfunction._Under_his_supervision_eleven_(11)_students_have_been_awarded_Ph.D.,_4_students_awarded_M.Phil._and_many_have_completed_post-graduate_dissertations._

 

He_is_engaged_in_teaching_the_Graduate,_Post-graduate_and_Doctoral_students_along_with_delivering_lectures_at_different_HRDCs_across_the_country_for_orientation_and_refresher_programs_for_teachers,_and_organised_Faculty_induction_programme_at_HRDC,_JNU_in_2023._He_represents_in_several_academic_and_administrative_committees,_selection_committees_and_has_held_various_responsible_positions_such_as_Assistant_controller_of_Examinations,_Deputy_Controller_of_Examinations,_Nodal_office_for_National,_Academic_depository,_and_convenor_and_coordinator_of_various_academic_bodies._

 

Research_Interest:_

 

Prof._Umesh_Chand_Singh_Yadav_carried_out_his_doctoral_studies_School_of_Life_Sciences_at_Jawaharlal_Nehru_University,_New_Delhi._He_pursued_his_research_work_in_the_area_of_‘Metabolic_Disorder_&_Inflammatory_Diseases_including_Cancer,_diabetes,_cardiovascular_diseases_and_lung_inflammatory_diseases_like_asthma_and_COPD’_as_a_Post_Doctoral_Fellow_and_faculty_member_at_the_University_of_Texas_Medical_Branch,_Galveston,_Texas,_USA._Prior_to_joining_the_‘Special_Centre_for_Molecular_Medicine’_in_the_year_2020,_he_was_working_as_Professor_at_the_Central_University_of_Gujarat,_Gandhinagar,_India._

His_research_interest_is_to_understand_biochemical_and_molecular_mechanism(s)_of_metabolic_disorder-induced_chronic_inflammatory_diseases_including_diabetic_and_cardiovascular_complications,_cancer,_asthma_and_COPD_through_cutting_edge_research,_and_discover_and_develop_potential_mechanism-based_molecular_medicine_for_clinical_intervention_and_therapy.

 

Projects:_

Completed:_

    Understanding_biochemical_and_molecular_link_between_obesity_and_Asthma
    _Role:_Principal_Investigator

    _Type:_Fellowship_Award_(Ramanujan_Fellowship)

    _Funding_Agency:_Department_of_Science_and_Technology_(DST),_Government_of_India

    _Status:_Completed_(Oct._2013-Oct._2018)

Regulation_of_endothelial_cells_dysfunction_by_Erk-5_in_metabolic_disorder     _Role:_Principal_Investigator

    _Type:_Early_Career_Research_Award

    _Funding_Agency:_SCIENCE_&_ENGINEERING_RESEARCH_BOARD_(SERB)/DST,_Government_of_India

    _Status:_Completed_(Oct._2016-Jan._2020)

SREBP-mediated_dysregulation_of_lipid_homeostasis_in_foam_cell_formation     _Role:_Principal_Investigator

    _Type:_Extramural

    _Funding_agency:_Gujarat_State_Biotechnology_Mission_(GSBTM),_DST,_Govt._of_Gujarat

    _Status:_Completed_(Oct._2016-Jan._2020)

Synthesis_of_natural_product_congeners_to_reinvigorate_the_investigation_of_their_chemistry_&_anti-inflammatory_pathogenesis_and_anti-carcinogenic_activities_     _Role:_Joint_Principal_Investigator

    _Type:_Extramural;_North-East_Twining_Grant

    _Funding_agency:_Dept._of_Biotechnology_(DBT)

    _Status:_Completed_(Jan._2017-_Jan._2020)

Ongoing:_

    Investigating_the_Role_of_RUNX2/Galectin-3_in_the_Pathogenesis_of_Cigarette_Smoke_Induced_Lung_Cancer:_Identification_of_Novel_Diagnostic_Biomarker_and_Potential_Therapeutic_Target_
    _Role:_Principal_Investigator_

    _Type:_Extramural_(EMR)

    _Funding_agency:_Dept._of_Biotechnology_(DBT)

    _Status:_Running_(July_2023-_July_2026)

Study_of_Epigenetic_Regulation_of_Erk5_by_Histone_Methylase,_Enhancer_of_Zeste_Homolog_(EZH)-2_during_oxLDL-induced_Endothelial_to_Mesenchymal_Transition_(EndMT)_     _Role:_Principal_Investigator_

    _Type:_Extramural_(CRG)

    _Funding_agency:_SERB

    _Status:_Not_started_yet_(Sept._2023-Sept._2026)

 

Collaborations:_

    Dr._Rajesh_Vasita,_Biomaterial_Engineering,_School_of_Life_Sciences,_Central_University_of_Gujarat,_Gandhinagar,_Gujarat,_India Prof_Rana_Pratap_Singh,_Cancer_Biology,_School_of_Life_Sciences,_JNU,_New_Delhi,_India. Dr._Ved_Prakash_Singh,_Department_of_Industrial_Chemistry,_Mizoram_University,_Aizawl,_Mizoram.
 

 

Selected_Publications:

 

    Rajput_PK,_Varghese_JF,_Srivastava_AK,_Kumar_U,_Yadav_UCS._Visfatin-induced_upregulation_of_lipogenesis_via_EGFR/AKT/GSK3β_pathway_promotes_breast_cancer_cell_growth._Cell_Signal._107:110686._Jul._2023._doi:_10.1016/j.cellsig.2023.110686. Sharma_JR,_Agraval_H,_Yadav_UCS._Cigarette_smoke_induces_epithelial-to-mesenchymal_transition,_stemness,_and_metastasis_in_lung_adenocarcinoma_cells_via_upregulated_RUNX-2/galectin-3_pathway._Life_Sci._318:121480._Apr_2023._doi:_10.1016/j.lfs.2023.121480. Rajput_PK,_Sharma_JR,_Yadav_UCS._Cellular_and_molecular_insights_into_the_roles_of_visfatin_in_breast_cancer_cells_plasticity_programs._Life_Sci._304:120706._Sept_2022._doi:_10.1016/j.lfs.2022.120706. Agraval_H,_Sharma_JR,_Dholia_N,_Yadav_UCS._Air-Liquid_Interface_Culture_Model_to_Study_Lung_Cancer-Associated_Cellular_and_Molecular_Changes._Methods_Mol_Biol._2413:133-144,_2022._doi:_10.1007/978-1-0716-1896-7_14. Agraval_H,_Sharma_JR,_Prakash_N,_Yadav_UCS._Fisetin_suppresses_cigarette_smoke_extract-induced_epithelial_to_mesenchymal_transition_of_airway_epithelial_cells_through_regulating_COX-2/MMPs/β-catenin_pathway._Chem_Biol_Interact._351:109771,_Jan_2022._doi:_10.1016/j.cbi.2021.109771. Varghese_JF,_Patel_R,_Singh_M,_Yadav_UCS._Fisetin_Prevents_Oxidized_Low-density_Lipoprotein-Induced_Macrophage_Foam_Cell_Formation._J_Cardiovasc_Pharmacol._78(5):e729-e737,_Nov_2021._doi:_10.1097/FJC.0000000000001096. Dholia_N,_Sethi_GS,_Naura_AS,_Yadav_UCS._Cysteinyl_leukotriene_D4_(LTD4)_promotes_airway_epithelial_cell_inflammation_and_remodelling._Inflamm_Res.70(1):109-126,_Jan._2021._doi:_10.1007/s00011-020-01416-z. 8.  _ Patel_R,_Varghese_JF,_Singh_RP,_Yadav_UCS._Induction_of_endothelial_dysfunction_by_oxidized_low-density_lipoproteins_via_downregulation_of_Erk-5/Mef2c/Klf2_signaling:_Amelioration_by_fisetin._Biochimie._11;_163:_152-162,_Jan_2019._doi._10.1016/j.biochi.2019.06.007 Varghese_JF,_Patel_R,_Yadav_UCS._Sterol_regulatory_element_binding_protein_(SREBP)_-1_mediates_oxidized_low-density_lipoprotein_(oxLDL)_induced_macrophage_foam_cell_formation_through_NLRP3_inflammasome_activation._Cellular_Signalling._53:_316-326,_Jan._2019._doi:_10.1016/j.cellsig.2018.10.020. Dholia_N_and_Yadav_UCS._Lipid_mediator_Leukotriene_D4-induces_airway_epithelial_cells_proliferation_through_EGFR/ERK1/2_pathway._Prostaglandins_Other_Lipid_Mediat._136:55-63,_May_2018._doi:_10.1016/j.prostaglandins.2018.05.003. Rani_V,_Deep_G,_Singh_RK,_Palle_K,_Yadav_UCS._Oxidative_stress_and_metabolic_disorders:_Pathogenesis_and_therapeutic_strategies,_Life_Sciences,_Available_online_3_February_2016,_ISSN_0024-3205,_Feb_2016._doi:_10.1016/j.lfs.2016.02.002. Yadav_UCS,_et_al.,_Aldose_reductase_regulates_acrolein-induced_cytotoxicity_in_human_small_airway_epithelial_cells._Free_Radic_Biol_Med._65C:15-25,_Dec._2013._doi:_10.1016/j.freeradbiomed.2013.06.008. Access_complete_PubMed_list_here:_https://pubmed.ncbi.nlm.nih.gov/?term=yadav+ucs&sort=date
 

Number_of_students_awarded/submitted_Ph._D.:_11

Number_of_Ph._D._students_currently_enrolled:_2   _ 

 

Professor_Shailja_Singh

 

Shailja_obtained_her_PhD_in_Biomedical_Sciences_at_the_University_of_Delhi._During_her_post-doctoral_tenure_at_the_International_Centre_for_Genetic_Engineering_and_Biotechnology_(ICGEB)_in_Delhi,_she_was_awarded_multiple_international_accolades,_including_the_Bill_&_Melinda_Gates_Foundation_Travel_Award_for_attending_malaria_conferences._Thereafter,_Shailja_joined_the_Department_of_Life_Sciences_at_Shiv_Nadar_Institution_of_Eminence_(SNIoE)_as_an_Associate_Professor,_where_she_received_prestigious_awards_such_as_the_Innovative_Young_Biotechnologist_Award_(IYBA)_from_the_Dept._of_Biotechnology_(DBT),_Govt._of_India,_and_the_Shiv_Nadar_University_Award_and_Indus_Foundation_Award_for_Research_Excellence._In_2016,_Shailja_joined_the_Special_Centre_for_Molecular_Medicine,_Jawaharlal_Nehru_University,_Delhi,_and_successfully_progressed_towards_attaining_the_position_of_a_full_professor._In_recognition_of_her_translational_research_in_Malaria_and_Kala-azar,_Shailja_has_been_honored_with_various_prestigious_awards,_including_the_Women_Bioscientist_Award_from_DBT_and_the_Biotechnology_Industry_Research_Assistance_Council-Gandhian_Young_Technological_Innovation_Award_(BIRAC-GYTIA)_from_the_Honorable_President_of_India._Shailja_currently_serves_as_an_expert_member_in_several_regulatory_bodies_within_the_university_and_the_Govt._of_India._With_25_years_of_research_experience,_her_expertise_lies_in_molecular_parasitology,_focusing_on_understanding_host-pathogen_interactions_in_diseases_like_malaria_and_Kala-azar._Scientific_contributions_from_Shailja’s_lab_have_been_published_in_esteemed_peer-reviewed_journals,_including_Nature_Communications,_EBioMedicine,_Proceedings_of_the_National_Academy_of_Sciences_(PNAS),_Advanced_Healthcare_Materials,_and_Cell_Death_Discovery,_among_others.

 

Research_Interest:

Shailja_exemplifies_a_highly_successful_homegrown_scientist_in_India._Her_research_group_focuses_on_understanding_the_fundamental_and_medicinal_aspects_of_human_malaria_and_leishmania_parasites._She_pioneered_the_identification_of_signaling_molecules_(Ca2+_and_cAMP)_and_signal_transduction_pathway_effectors_(CDPK1,_CN,_PAP,_DGK,_PAT,_PLPs,_etc.)_involved_in_malaria_parasite_invasion_and_egress._This_groundbreaking_work_has_paved_the_way_for_innovative_anti-malarial_medications._Shailja’s_research_group_has_significantly_contributed_to_translational_research,_particularly_in_anti-malarial_and_anti-Leishmanial_inhibitor_screening_and_development._Recognized_by_Biotechnology_Industry_Research_Assistance_Council_(BIRAC)-Gandhian_Young_Technological_Innovation_(GYTI)_awards,_she_has_developed_breakthrough_drug_discovery_tools._Furthermore,_she_introduced_a_nanomedicine-based_approach_using_surface-coated_iron-oxide-nanoparticles_to_enhance_the_efficacy_of_the_age-old_anti-malarial_medication,_Artesunate._In_collaboration_with_Institut_Pasteur,_France,_she_discovered_that_the_patatin-like_phospholipase_that_regulates_gametocyte_egress_has_the_potential_to_prevent_malaria_transmission._Notably,_her_recent_discovery_of_a_host_erythrocyte_micro-RNA,_miR-197-5p,_revealed_an_unusual,_nucleotide-based_therapy_and_inspired_a_novel_strategy_for_generating_anti-malarial_molecular_pharmaceuticals._Her_research_group_has_also_identified_several_parasite_chaperones,_such_as_Prefoldins,_Cold_shock_protein,_and_Prohibitins,_while_exploiting_host_proteins_like_SphK1,_HSP-70,_PMCA4,_G6PD,_LanCl2,_Kell,_and_NMDA_for_antimalarial_drug_development.

 

Ongoing_Projects

1._Title: “Evaluation_of_the_role_of_S1P_in_Tribal_Plasmodium_falciparum_malaria_patients_for_its_potential_therapeutic_consideration” Brief_abstract: This_project_aims_to_establish_the_role_of_Sphingosine-1-Phosphate_(S1P)_in_severe_human_malaria_of_various_clinical_manifestations,_particularly_in_adult_patients._Besides,_the_study_will_investigate_whether_genetic_variations_in_the_S1P_pathway_contribute_to_the_clinical_severity_of_malaria._Further,_it_will_also_explore_the_influence_of_S1P_on_critical_pathogenic_processes_in_severe_malaria,_such_as_endothelial_dysfunction,_inflammation,_and_NO_production._Overall,_the_findings_will_provide_clues_for_the_prognostic_implication_of_S1P_in_severe_P._falciparum_malaria_and_could_support_its_use_as_an_adjunctive_therapeutic_in_severe_malaria_cases. Funding_agency: Indian_Council_of_Medical_Research_(ICMR),_Government_of_India File_no.: NER/84/2022-ECD-I

2._Title: “A_comprehensive_characterization_of_key_organellar_metabolic_transporters_in_Apicomplexa_to_enter_in_the_drug_discovery_pipeline” Brief_abstract: This_research_addresses_the_critical_need_for_the_development_of_novel_and_effective_antimalarial_compounds_with_limited_off-target_effects._Surprisingly,_there_is_little_redundancy_in_the_Plasmodium_'Transportome'_and_substantial_evidence_that_membrane_transporter_proteins_are_likely_to_serve_as_therapeutic_targets._Our_integrative_strategy,_which_combines_biochemical,_biophysical,_and_molecular_approaches,_will_allow_us_to_identify_small_molecule_candidates_against_the_transport_of_mono-carboxylates,_which_is_critical_for_parasite_survival._The_ultimate_goal_of_the_project_is_to_determine_whether_these_transporters_have_antimalarial,_transmission-blocking,_or_preventive_potential. Funding_agency: Indo-Department_of_Biotechnology_(DBT),_Government_of_India_and_Swiss_National_Science_Foundation_(SNSF),_Switzerland File_no.: IC-12044(11)/10/2021-ICD-DBT

3._Title: “A_multi-targeted_approach_encompassing_fundamental_and_applied_studies_towards_drug_discovery_for_Leishmaniasis” Brief_abstract: Leishmaniasis_is_one_of_the_major_neglected_tropical_diseases,_for_which_no_vaccines_exist._Chemotherapy_is_hampered_by_limited_efficacy_coupled_with_the_development_of_resistance_and_other_side_effects._The_Leishmania/macrophage_interaction_provides_an_excellent_example_of_co-evolution_that_promotes_parasite_survival._Conceivably,_interfering_with_these_processes_represents_a_promising_novel_strategy_against_Leishmania._Towards_this,_a_multi-targeted_approach_is_required_to_get_a_better_understanding_of_these_host-pathogen_interactions_to_develop_a_drug._Here,_we_propose_to_target_four_essential_mechanisms_of_Leishmania_pathogenesis_to_block_motility,_invasion,_growth,_and_sustenance_inside_the_host_macrophages. Funding_agency: Science_and_Engineering_Research_Board_(SERB),_Department_of_Science_&_Technology_(DST),_Government_of_India File_no.: IPA/2020/000007

NUMBER_OF_STUDENTS_AWARDED/SUBMITTED_Ph.D.:_18

NUMBER_OF_Ph.D._STUDENTS_CURRENTLY_ENROLLED:_06

 

Selected_Publications:_148_

Papers_in_peer-reviewed_journals_(in_reverse_chronological_order;_*Corresponding_author)

  1. Sadat_Shafi,_Sonal_Gupta,_Ravi_Jain,_Rumaisha_Shoaib,_Akshay_Munjal,_Preeti_Maurya,_Purnendu_Kumar,_Abul_Kalam_Najmi,_Shailja_Singh*._Tackling_the_emerging_Artemisinin-resistant_malaria_parasite_by_modulation_of_defensive_oxido-reductive_mechanism_via_nitrofurantoin_repurposing._Biochemical_Pharmacology;_Sept._2023,215,_115756._doi:_10.1016/j.bcp.2023.115756
  2. Atul,_Preeti_Chaudhary,_Swati_Gupta,_Rumaisha_Shoaib,_Rahul_Pasupureddy,_Bharti_Goyal,_Bhumika_Kumar,_Om_Prakash_Singh,_Rajnikant_Dixit,_Shailja_Singh,_Mymoona_Akhter,_Neera_Kapoor,_Veena_Pande,_Soumyananda_Chakraborti,_Kapil_Vashisht,_Kailash_C_Pandey._Artemisinin_resistance_in_P._falciparum:_Probing_the_interacting_partners_of_Kelch13_protein_in_parasite._Journal_of_Global_Antimicrobial_Resistance;_2023_Aug_24;_In_press.
  3. Kashish_Azeem,_Mofieed_Ahmed,_Amad_Uddin,_Shailja_Singh,_Rajan_Patel,_Mohammad_Abid._Comparative_Investigation_on_Interaction_of_Potent_Antimalarials_with_Human_Serum_Albumin_via_Multispectroscopic_and_Computational_Approaches._Luminescence._2023_Aug_31._doi:_10.1002/bio.4590.
  4. Miklós_Bege,_Vigyasa_Singh,_Neha_Sharma,_Nóra_Debreczeni,_Ilona_Bereczki,_Poonam,_Pál_Herczegh,_Brijesh_Rathi*,_Shailja_Singh*,_Anikó_Borbás*._In_vitro_and_in_vivo_antiplasmodial_evaluation_of_sugar-modified_nucleoside_analogues._Sci_Rep._2023_Jul_28;13(1):12228._doi:_10.1038/s41598-023-39541-4.
  5. Ravi_Ranjan_Kumar,_Ravi_Jain,_Sabir_Akhtar,_Nidha_Parveen,_Arabinda_Ghosh,_Veena_Sharma,_Shailja_Singh*._Characterization_of_thiamine_pyrophosphokinase_of_vitamin_B1_biosynthetic_pathway_as_a_drug_target_of_Leishmania_donovani._J_Biomol_Struct_Dyn._2023_Jun_23;1-17._doi:_10.1080/07391102.2023.2227718._Online_ahead_of_print.
  6. Kashish_Azeem,_Iram_Irfan,_Qudsia_Rashid,_Shailja_Singh,_Rajan_Patel,_Mohammad_Abid._Recent_Updates_on_Interaction_Studies_and_Drug_Delivery_of_Antimalarials_with_Serum_Albumin_proteins._Curr_Med_Chem._2023_May_9._doi:_10.2174/0929867330666230509121931.
  7. Deepika_Kannan,_Nishant_Joshi,_Sonal_Gupta,_Soumya_Pati,_Souvik_Bhattacharjee,_Gordon_Langsley_&_Shailja_Singh*._Cytoprotective_autophagy_as_a_pro-survival_strategy_in_ART-resistant_malaria_parasites._Cell_Death_Discov._2023_May_13;9(1):160._doi:_10.1038/s41420-023-01401-5.
  8. Sashi_Bhusan_Ojha,_Raj_Kumar_Sah,_Evanka_Madan,_Ruby_Bansal,_Shaktirekha_Roy,_Shailja_Singh*_&_Gunanidhi_Dhangadamajhi._Cuscuta_reflexa_Possess_Potent_Inhibitory_Activity_Against_Human_Malaria_Parasite:_An_In_Vitro_and_In_Vivo_Study._Curr_Microbiol_80,_189_(2023)._https://doi.org/10.1007/s00284-023-03289-x.
  9. Ifeoma_Ezenyi,_Evanka_Madan,_Jhalak_Singhal,_Ravi_Jain,_Amrita_Chakrabarti,_Gajala_Deethamvali_Ghousepeer,_Ramendra_Pati_Pandey,_Ngozichukwuka_Igoli,_John_Igoli,_Shailja_Singh*._Screening_of_traditional_medicinal_plant_extracts_and_compounds_identifies_a_potent_antileishmanial_diarylheptanoid_from_Siphonochilus_aethiopicus._J_Biomol_Struct_Dyn._2023_May_18;1-15._doi:_10.1080/07391102.2023.2212779.
  10. Monika_Saini,_Che_Julius_Ngwa,_Manisha_Marothia,_Pritee_Verma,_Shakeel_Ahmad,_Jyoti_Kumari,_Sakshi_Anand,_Vandana_Vandana,_Bharti_Goyal,_Soumyananda_Chakraborti,_Kailash_C._Pandey,_Swati_Garg,_Soumya_Pati,_Anand_Ranganathan,_Gabriele_Pradel,_Shailja_Singh*._Characterization_of_Plasmodium_falciparum_prohibitins_as_novel_targets_to_block_infection_in_humans_by_impairing_the_growth_and_transmission_of_the_parasite._Biochem_Pharmacol._2023_Jun;212:115567._doi:_10.1016/j.bcp.2023.115567.
  11. Ankita_Behl,_Rumaisha_Shoaib,_Fernando_De_Leon,_Geeta_Kumari,_Monika_Saini,_Evanka_Madan,_Vikash_Kumar,_Harshita_Singh,_Jyoti_Kumari,_Preeti_Maurya,_Swati_Garg,_Prakash_Chandra_Mishra,_Christoph_Arenz,_Shailja_Singh*._Targeting_essential_plasmodium_cold_shock_protein_to_block_growth_and_transmission_of_malaria_parasite._iScience._2023_Apr_11;26(5):106637._doi:_10.1016/j.isci.2023.106637.
  12. Nidhi_Kirtikumar_Bub,_Sakshi_Anand,_Swati_Garg,_Vishal_Saxena,_Dhanabala_Subhiksha_Rajesh_Khanna,_Deeptanshu_Agarwal,_Sanjay_Kumar_Kochar,_Shailja_Singh*,_Shilpi_Garg._Plasmodium_Iron-Sulfur_[Fe-S]_cluster_assembly_protein_Dre2_as_a_plausible_target_of_Artemisinin:_Mechanistic_insights_derived_in_a_prokaryotic_heterologous_system._Gene._2023_Mar_28;869:147396._doi:_10.1016/j.gene.2023.147396.
  13. Raj_Kumar_Sah,_Sakshi_Anand,_Waseem_Dar,_Ravi_Jain,_Geeta_Kumari,_Evanka_Madan,_Monika_Saini,_Aashima_Gupta,_Nishant_Joshi,_Rahul_Singh_Hada,_Nutan_Gupta,_Soumya_Pati,_Shailja_Singh*._Host-Erythrocytic_Sphingosine-1-Phosphate_Regulates_Plasmodium_Histone_Deacetylase_Activity_and_Exhibits_Epigenetic_Control_over_Cell_Death_and_Differentiation._Microbiol_Spectr._2023_Feb_6;e0276622._doi:_10.1128/spectrum.02766-22.

 

Dr._Souvik_Bhattacharjee,_Associate_Professor

Dr._Souvik_Bhattacharjee_carried_out_his_doctoral_studies_the_CSIR-Institute_of_Microbial_Technology,_Chandigarh._He_pursued_his_research_work_in_the_area_of_‘Protein_trafficking_in_malaria-infected_erythrocytes_and_the_mechanism_of_artemisinin-resistance’,_firstly_as_a_post-doctoral_fellow_at_Northwestern_University,_Chicago,_and_then_as_Research_Assistant_Professor_at_the_University_of_Notre_Dame,_Indiana._Prior_to_joining_the_‘Special_Centre_for_Molecular_Medicine’_in_2015,_he_was_working_in_artemisinin-resistance_mechanisms_Plasmodium_falciparum_at_the_University_of_Notre_Dame,_USA.  

Research_Interest:_

The_research_in_my_laboratory_is_directed_towards_the_elucidation_of_targeting_signals_that_sort_proteins_destined_for_localization_within_the_different_compartments_in_the_host-pathogen_interface._Primarily,_we_are_looking_in_Plasmodium_falciparum_as_the_human_pathogen,_and_Phytophthora_infestans_as_the_plant_pathogen._We_trace_the_evolutionary_convergence_in_the_virulent_protein_trafficking_mechanisms_across_pathogens_of_different_origin_and_we_characterize_essential_motifs_using_transgenic_approaches. _Our_other_area_of_interest_involves_understanding_the_contribution_of_host_factors_towards_the_development_of_artemisinin-resistance_in_P._falciparum.

 

Ongoing_Projects:

  1. Translating_the_Phylogenetic_affinities_between_a_plant_pathogenic_oomycete_Phytophthora_infestans_and_a_human_pathogen_Plasmodium_falciparum_to_reveal_evolutionary_convergence_in_virulence_secretion_using_in-silico,_proteomic_and_metabolomic_approaches._(DST-SERB_CRG/2021/000238).
  2. Elucidating_the_role_of_clinical_anemia_in_the_induction_of_artemisinin_resistance_in_Plasmodium_falciparum._(ICMR;_58/25/2020/PHA/BMS).
  3. Unravelling_the_molecular_mechanisms_underlying_human_ABO_blood_type_preference_by_the_virulence_RIFIN_variants_in_Plasmodium_falciparum-infected_erythrocyte_rosettes._(MoE_STARS_Project_ID:_2023-0111).

 

Collaborations:_

IIT-Delhi;_RCB,_Faridabad,_University_of_Notre_Dame,_USA;

 

Selected_Publications:

  1. Hari_Madhav,_Tarosh_S._Patel,_Zeba_Rizvi,_G._Srinivas_Reddy,_Abdur_Rahman,_Md._Ataur_Rahman,_Saiema_Ahmedi,_Sadaf_Fatima,_Kanika_Saxena,_Nikhat_Manzoor,_Souvik_Bhattacharjee,_Bharat_C._Dixit,_Puran_Singh_Sijwali,_Nasimul_Hoda._Development_of_diphenylmethylpiperazine_hybrids_of_chloroquinoline_and_triazolopyrimidine_using_Petasis_reaction_as_new_cysteine_proteases_inhibitors_for_malaria_therapeutics._European_Journal_of_Medicinal_Chemistry._Volume_258,_5_October_2023,_115564.
  2. Kannan_D,_Joshi_N,_Gupta_S,_Pati_S,_Bhattacharjee_S,_Langsley_G,_Singh_S._Cytoprotective_autophagy_as_a_pro-survival_strategy_in_ART-resistant_malaria_parasites._Cell_Death_Discov._2023_May_13;_9(1):160._doi:_10.1038/s41420-023-01401-5.
  3. Pal_K,_Raza_Md._K,_Legac_J,_Rahman_A,_Manzoor_S,_Bhattacharjee_S,_Rosenthal_PJ_and_Hoda_N._Identification,_in-vitro_anti-plasmodial_assessment_and_docking_studies_of_series_of_tetrahydrobenzothieno[2,3-d]pyrimidine-acetamide_molecular_hybrids_as_potential_antimalarial_agents._European_Journal_of_Medicinal_Chemistry._Volume_248,_15_February_2023,_115055
  4. Chaurasiya_A,_Kumari_G,_Garg_S,_Shoaib_R,_Anam_Z,_Joshi_N,_Kumari_J,_Singhal_J,_Singh_N,_Kaushik_S,_Kahlon_AK,_Dubey_N,_Maurya_MK,_Srivastava_P,_Marothia_M,_Joshi_P,_Gupta_K,_Saini_S,_Das_G,_Bhattacharjee_S,_Singh_S,_Ranganathan_A._Targeting_Artemisinin-Resistant_Malaria_by_Repurposing_the_Anti-Hepatitis_C_Virus_Drug_Alisporivir._Antimicrob_Agents_Chemother._2022_Dec_20;66(12):_e0039222._doi:_10.1128/aac.00392-22._Epub_2022_Nov_14._PMID:_36374050
  5. Goel_N,_Dhiman_K,_Kalidas_N,_Mukhopadhyay_A,_Ashish_F,_Bhattacharjee_S._Plasmodium_falciparum_Kelch13_and_its_artemisinin-resistant_mutants_assemble_as_hexamers_in_solution:_a_SAXS_data-driven_modelling_study._FEBS_J._2022_Jan_28._doi:_10.1111/febs.16378._Online_ahead_of_print._PMID:_35092154.
  6. Kumar_T,_Maitra_S,_Rahman_A,_Bhattacharjee_S._A_conserved_guided_entry_of_tail-anchored_pathway_is_involved_in_the_trafficking_of_a_subset_of_membrane_proteins_in_Plasmodium_falciparum._PLoS_Pathog._2021_Nov_15;17(11):_e1009595._doi:_10.1371/journal.ppat.1009595.
  7. Nayak_A,_Saxena_H,_Bathula_C,_Kumar_T,_Bhattacharjee_S,_Sen_S_and_Gupta_A._Diversity‑oriented_synthesis_derived_indole_based_spiro_and_fused_small_molecules_kills_artemisinin‑resistant_Plasmodium_falciparum._Malar_J_(2021)_20:100._https://doi.org/10.1186/s12936-021-03632-2.
  8. Kannan_D,_Yadav_N,_Ahmad_S,_Namdev_P,_Bhattacharjee_S,_Lochab_B_and_Singh_S._Pre-clinical_study_of_iron_oxide_nanoparticles_fortified_artesunate_for_efficient_targeting_of_malarial_parasite._EBioMedicine._2019_Jul;_45:261-277._doi:_10.1016/j.ebiom.2019.06.026.
  9. Bhattacharjee_S,_Coppens_I,_Mbengue_A,_Suresh_N,_Ghorbal_M,_Slouka_Z,_Safeukui_I,_Tang_HY,_Speicher_DW,_Stahelin_RV,_Mohandas_N,_Haldar_K._Remodeling_of_the_malaria_parasite_and_host_human_red_cell_by_vesicle_amplification_that_induces_artemisinin_resistance._Blood._2018_Mar_15;131(11):1234-1247.
  10. Haldar_K,_Bhattacharjee_S,_Safeukui_I._Drug_resistance_in_Plasmodium._Nat_Rev_Microbiol._2018_Mar;16(3):156-170._doi:_10.1038/nrmicro.2017.161._Epub_2018_Jan_22._Review.
  11. Alassane_Mbengue,_Souvik_Bhattacharjee*,_Trupti_Pandharkar,_Haining_Liu,_Guillermina_Estiu,_Robert_V._Stahelin,_Shahir_Rizk,_Dieudonne_L._Njimoh,_Yana_Ryan,_Kesinee_Chotivanich,_Chea_Nguon,_Mehdi_Ghorbal,_Jose-Juan_Lopez-Rubio,_Michael_Pfrender_,_Scott_Emrich,_Narla_Mohandas,_Arjen_M._Dondorp,_Olaf_Wiest_and_Kasturi_Haldar._A_molecular_mechanism_of_artemisinin_resistance_in_Plasmodium_falciparum_malaria._Nature_520,_683–687_(30_April_2015)._*Equal_contributing_author._ 
  12. Bhattacharjee_S,_Stahelin_RV,_Speicher_KD,_Speicher_DW,_Haldar_K._Endoplasmic_Reticulum_PI(3)P_lipid_binding_targets_malaria_proteins_to_the_host_cell._Cell._2012_Jan_20;_148(1-2):_201-12.

  Number_of_students_awarded/submitted_Ph._D.:_04 Number_of_Ph._D._students_currently_enrolled:_04

 

Dr._Vijay_Pal_Singh_Rawat,_Associate_Professor

Dr_Vijay_Pal_Singh_Rawat_did_his_Ph.D_in_human_biology _from_Ludwig_Maximilians_University_(LMU),_Munich,_Germany_with_Summa_cum_laude_("with_highest_honor")._During_his_doctoral_studies_he_identified_and_characterized_the_leukemia_specific_genetic_and_molecular_alterations_and_their_functional_role_in_leukemia_initiation_and_progression. _Dr_Rawat_demonstrated_for_the_first_time_that_the_activation_of_a_proto-oncogene_-_in_this_case_CDX2_-_by_a_chromosomal_translocation_can_be_the_key_step_in_myeloid_leukemogenesis,_even_if_the_fusion_gene_is_generated_and_expressed_in_parallel._Before_joining_JNU,_Dr_Rawat_worked_as_an_Assistant_Professor_and_Principal_Scientist_at_University_of_Ulm,_Germany_and_Institute_of_Experimental_Cancer_Research,_University_Hospital_Ulm,_Ulm,_Germany,_where_his_research_group_analysed_the_functional_role_of_stem_cell_regulatory_genes,_epigenetic_factors_and_recently_discovered_epigenetic_marks_5hmC_in_cancer_development,_progression_and_identify_potential_drug_target_for_leukaemia_treatment._Dr._Rawat_joined_the_Special_Centre_for_Molecular_Medicine,_JNU_in_January_2021.

 

Research_Interest:

Dr._Rawat_is_working_on_cancer,_cancer_stem_cell_and_hematopoiesis._The_key_focuses_of_Dr_Rawat’s_research_is_a)_Identifying_and_characterizing_cancer-patient-specific_genetic/epigenetic_and_molecular_alterations_and_their_functional_role_in_cancer_initiation_and_progression,_b)_Establishing_mouse_and_humanized_mouse_models_of_leukemia_by_using_stem/progenitors_transplantation_model_to_identify_novel_drug_targets_for_leukemia_treatment_and_to_assay_drug_screening._c)_Characterization_of_the_functional_role_of_epigenetic_factors_in_stem_cell_ageing_and_cancer_stem_cell_(CSC)._Dr_Rawat_has_profound_experience_in_establishing_cancer_models_using_lentivirus/retrovirus_based_vector_system_and_lentiviral-mediated_CRISPR/Cas9_genome_editing_system_which_is_evident_from_his_research_work_published_in_high_ranking_international_journals_(PNAS,_Blood,_Leukemia,_JCI,_Cancer_Cell_etc,_please_see_the_CV_for_details)._Currently,_Dr_Rawat’s_laboratory_is_interested_in_the_following_areas-

A.  _ Identification_and_characterization_the_functional_role_of_DNA_demethylating_enzymes_in_acute_leukemia_and_solid_cancer

B.  _ Determining_the_role_of_epigenetic_modifier_Tet_dioxygenase_and_5-hydroxymethyl-cytosine_modifications_in_normal_stem_cell_ageing_and_cancer_stem_cell

C.  _ Characterization_of_the_role_of_small_non-coding_RNA_and_RNA_binding_proteins_in _cancer_development_and_normal_stem_cells

D.  _ Development_of_strategies_to_antagonize_the_leukemogenic_potential_of_the_homeobox_genes_in_acute_leukemia  

Ongoing_Projects:

  1. Title:_Characterizing_the_functional_role_of_DNA_binding_domain_lacking_novel_alternative_splice_form_of_TET1_in_acute_myeloid_leukemia,_leukemic_stem_cells_and_can_it_be_chemically_targeted._Funding_agency:_ICMR
  2. Title:_Uncovering_the_novel_oncogenic_role_of_Thymine_DNA_Glycosylase_epigenetic_function_in_acute_leukemia_is_a_potential_therapeutic_target_for_acute_leukemia_treatment. _Funding_agency:_SERB-DST
  3. Title:_Characterizing_the_oncogenic_role_of_TET3_and_associated_epigenetic_marks_5-hydorxymethylcytosine_in_gene_regulation_and_pathogenesis_of_acute_myeloid_leukemia._Funding_agency:_SERB-DST

 

Collaborations:_

Prof._Ritu_Gupta,_AIIMS,_New_Delhi._Prof._Rana_P_Singh,_SLS,_JNU,_New_Delhi._Prof._Dr._med._Christian_Buske,_Director,_Institute_of_Experimental_Cancer_Research,_Ulm,_Germany.

Prof._Dr._med._Michaela_Feuring, _Department_of_Internal_Medicine_III,_University_Hospital_Ulm,_Germany

 

Selected_Publications:_(in_last_five_years)

  1. Bamezai_S,_Pulikkottil_AJ,_Yadav_T,_Naidu_VM,_Mueller_J,_Mark_J,_Mandal_T,_Feder_KA,_Lehle_S,_Song_C,_Rosler_R,_Wiese_S,_Hoell_JI,_Kloetgen_A,_Karsan_A,_Kumari_A,_Wojenski_L,_Sinha_AU,_Gonzalez-Menendez_I,_Quintanilla-Martinez_L,_Donato_E,_Trumpp_A,_Kruse_E,_Hamperl_S,_Zou_L,_Rawat_VPS_*,_Buske_C*._A_non-canonical_enzymatic_function_of_PIWIL4_maintains_genomic_integrity_and_leukemic_growth_in_AML._Blood._July_2023,_6;142(1):90-105._(IF:_25)
  2. Pulikkottil_AJ _Bamezai_S,_Ammer_T,_Mohr_F,_Feder_K,_Vegi_NM,_Mandal_T,_Kohlhofer_U, _Quintanilla-Martínez_L,_Singh_A,_Buske_C*,_Rawat_VPS*._TET3_promotes_AML_growth_and_epigenetically_regulates_glucose_metabolism_and_leukemic_stem_cell_associated_pathways._Leukemia,_Feb._2022,_36(2):416-425.._(IF:_12.5)
  3. Bamezai_S,_Demir_D,_Pulikkottil_A,_Ciccarone_F,_Fischbein_E,_Sinha_A,_Borga_C,_Kronnie_Gt,_Meyer_LH,_Mohr_F,_Götze_M,_Caiafa_P,_Debatin_KM,_Döhner_K,_Döhner_H,_Menendez_I,_Quintanilla-Martínez_L,_Herold_T,_Jeremias_I,_Feuring-Buske_M,_Buske_C*,_Rawat_VPS*._TET1_acts_as_pro-leukemic_factor_in_human_T-cell_acute_lymphoblastic_leukemia_and_can_be_antagonized_via_PARP_inhibition._Leukemia,_Feb;35(2):389-403,_2021._(IF:_12.5)
  4. Rawat_VPS*≠,_Götze_M≠,_Rasalkar_A,_Vegi_NM,_Ihme_S,_Thoene_S,_Pastore_A,_Bararia_D,_Döhner_H,_Döhner_K,_Feuring-Buske_M,_Quintanilla-Fend_L,_Buske_C*._The_microRNA_miR-196b_acts_as_tumor_suppressor_in_Cdx2_driven_acute_myeloid_leukemia._Haematologica._2020_Jun;105(6):e285-e289_(_IF:_10.2)
  5. Thoene_S,_Mandal_T,_Vegi_NM,_Quintanilla-Martinez_L,_Rösler_R,_Wiese_S,_Metzeler_KH,_Herold_T,_Haferlach_T,_Döhner_K,_Döhner_H,_Schwarzmüller_L,_Klingmüller_U,_Buske_C,_Rawat_VPS*,_Feuring-Buske_M*._The_ParaHox_gene_Cdx4_induces_acute_erythroid_leukemia_in_mice._Blood_Adv._2019_Nov_26;3(22):3729-3739._(_IF:_7.63)

 

Number_of_students_awarded/submitted_Ph._D.:_2

Number_of_Ph._D._students_currently_enrolled:_4

 

 

Dr._Saima_Aijaz,_Assistant_Professor

Dr._Saima_Aijaz_received_her_Ph.D_degree_from_the_Indian_Institute_of_Science,_Bangalore,_India_where_she_worked_on_the_outer_capsid_protein_of_rotavirus_to_evaluate_its_potential_as_a_recombinant_vaccine_candidate._During_her_post-doctoral_work_at_University_College_London_(United_Kingdom),_she_worked_on_the_functional_characterization_of_proteins_associated_with_epithelial_tight_junctions._After_joining_SCMM,_Dr._Aijaz_started_investigating_the_effect_of_tight_junction_disruption_on_the_pathogenesis_of_intestinal_infections_caused_by_Enteropathogenic_E._coli_as_well_as_in_rare_diseases_such_as_retinitis_pigmentosa_type_12.

 

Research_Interest:_

1._Regulation_of_the_tight_junction_barrier_in_Enteropathogenic_E._coli_infections 2._Disruption_of_the_Tight_Junctions_in_Retinitis_Pigmentosa_Type-12

Tight_junctions_seal_adjacent_epithelial_cells_to_selectively_regulate_paracellular_permeability_and_prevent_the_intermixing_of_apical_and_basolateral_proteins_of_the_plasma_membrane._Regulation_of_paracellular_permeability_is_a_critical_function_of_tight_junctions_and_increased_permeability_is_associated_with_diverse_disease_conditions_ranging_from_bacterial_and_viral_infections_to_cancer_and_metastasis._Research_in_the_laboratory_is_focused_on_the_molecular_mechanisms_that_regulate_the_disruption_of_tight_junctions_in_epithelial_cells_in_response_to_pathologic_stimuli._In_one_approach,_Enteropathogenic_E._coli_(EPEC)_is_used_as_a_model_system_to_study_the_regulation_of_paracellular_permeability._EPEC_disrupts_epithelial_tight_junctions_and_is_a_leading_cause_of_diarrhea_in_the_developing_world_causing_significant_morbidity_and_mortality_due_excessive_permeability_of_water_and_electrolytes_through_the_intestinal_tight_junctions._However,_the_underlying_mechanisms_that_cause_the_drastic_increase_in_permeability_through_intestinal_junctions_have_not_been_elucidated_yet._The_laboratory_is_engaged_in_the_identification_of_tight_junction-based_signaling_pathways_that_regulate_the_EPEC-mediated_leakage_through_tight_junctions_with_the_ultimate_goal_of_identifying_molecules_to_block/reverse_this_leakage._In_another_approach,_work_is_being_carried_out_to_understand_how_defects_in_the_tight_junctions_of_retinal_pigmented_epithelia_prevent_the_normal_functioning_of_the_retina_in_retinitis_pigmentosa_type_12._

 

Ongoing_Projects:_

1._Restoration_of_the_intestinal_barrier_in_Enteropathogenic_E._coli_infections:_Lysosome_and_cytoskeleton_pathways_as_novel_drug_targets_(PI)._(2020-2024)._Funded_by_MHRD-STARS

The_aim_of_this_project_is_to_identify_whether_modulation_of_the_host_cell_lysosomal_machinery_can_prevent_leakage_through_intestinal_tight_junctions.

2._Mechanisms_of_retinal_degeneration_in_Retinitis_Pigmentosa_Type_12:_Role_of_the_Crumbs_homology_proteins-1_and_-2_(PI)._(2020-2023)._Funded_by_ICMR.

This_project_is_designed_to_understand_how_defects_in_the_Crumbs_homology_proteins-1_and_-2_cause_retinitis_pigmentosa_type_12.

 

Selected_Publications:

    Aijaz,_S._(2020)._Tracing_the_origins_of_the_novel_coronavirus_SARS-CoV-2._JNU-ENVIS._25_(1):19-20 Singh_A.P.,_Sharma_S.,_Pagarware_K.,_Siraji_R._A.,_Ansari_I.,_Mandal_A.,_Walling_P._and _Aijaz_S._(2018)._Enteropathogenic_E._coli_effectors_EspF_and_Map_independently_disrupt_tight_junctions_through_distinct_mechanisms_involving_transcriptional_and_post-transcriptional_regulation._Scientific_Reports_8:_3719 Singh_AP_and_Aijaz_S_(2016)._Enteropathogenic_E._coli:Breaking_the_intestinal_tight_junction_barrier._F1000Research._4:231. Singh_AP_and_Aijaz_S_(2015)._Generation_of_a_MDCK_cell_line_with_constitutive_expression_of_the_Enteropathogenic_E._coli_effector_protein_Map_as_an_in_vitro_model_of_pathogenesis._Bioengineered._6(6):_335-341. Nie_M,_Aijaz_S,_Leefa_Chong_San_IV,_Balda_MS,_Matter_K._(2009)._The_Y-box_factor_ZONAB/DbpA_associates_with_GEF-H1/Lfc_and_mediates_Rho-stimulated_transcription._EMBO_Reports_10:1125-1131. Aijaz_S,_Sanchez-Heras_E,_Balda_MS,_Matter_K._(2007)._Regulation_of_tight_junction_assembly_and_epithelial_morphogenesis_by_the_heat_shock_protein_Apg-2._BMC_Cell_Biology,_8:49. Aijaz_S,_Balda_MS,_Matter_K._(2006)_Tight_junctions:_Molecular_architecture_and_function._International_Reviews_in_Cytology;_248:_261-298. Matter_K,_Aijaz_S,_Tsapara_A,_Balda_MS._(2005)_Mammalian_tight_junctions_in_the_regulation_of_epithelial_differentiation_and_proliferation._Current_Opinion_in_Cell_Biology;_17(5):_453-458. Aijaz_S,_D'Atri_F,_Citi_S,_Balda_MS,_Matter_K._(2005)._Binding_of_GEF-H1_to_the_tight_junction-associated_adaptor_cingulin_results_in_inhibition_of_Rho_signalling_and_G1/S_phase_transition._Developmental_Cell;_8(5):_777-786.

Number_of_students_awarded_Ph.D:_7

Number_of_Ph.D_students_currently_enrolled:_3

 

Dr._Dipankar_Ghosh,_Assistant_Professor

 

 

Dipankar_Ghosh_carried_out_his_doctoral_studies_in_Jadavpur_University,_Kolkata._He_pursued_his_postdoctoral_research_in_Immunology_and_Infectious_Diseases_in_the_Cleveland_Clinic,_Ohio_USA,_where_their_group_discovered_the_post-translational_processing_and_functions_of_human_defensin_5_(HD5)_in_innate_immune_response._For_this_he_won_the_Berlin_Bumpus_Investigator_award_in_the_USA._Subsequently_he_worked_in_the_Indian_Institute_of_Technology_(IIT),_Kharagpur,_before_joining_SCMM.

 

Research_Interests:

We_work_on_early_host-microbe_interactions,_that_ultimately_define_the_predisposition_and/or_outcomes_of_diseases._The_human_epithelial_surface_is_continuously_exposed_to_microbes._This_epithelium_presents_the_first_line_of_defence_against_external_threats._This_defence_is_not_physical_barrier_alone;_but_extremely_complex_array_of_receptors,_signalling_cascades_and_effectors_that_are_key_to_innate_immunity. _We_are_interested_to_understand_how_epithelial_and_innate_immune_cells_interact_with_themselves_and_bacteria_to_exert_this_innate_immunity._For_this_we_study_nutrition,_microbiota_and_biofilms_with_specific_emphasis_on_Quorum_signalling_and_the_cross_talk_with_innate_immune_determinants._These_studies_help_to_contribute_to_the_understanding_of_lethal_multiple_drug_resistant_nosocomial_infections_and_sepsis,_especially_neonatal_sepsis_which_kills_thousands_of_babies_in_early_life._We_are_currently_part_of_the_National_Neonatal_Sepsis_Research_Consortium_(DBT),_one_of_the_largest_research_programs_in_India_in_collaboration_with_AIIMS,_TSHTI,_IGIB,_NII_and_ICGEB.

 

Ongoing_Projects:_1

 

Collaborations:

Prof._Sanjoy_K._Bhattacharya,_Bascom_Palmer_Eye_Institute,_Florida,_U.S.A_(Lipidomics).

National_Collaborations: The_Indian_Neonatal_Sepsis_Consortium_(AIIMS-Del,_NII,_ICGEB,_TSHTI,_IGIB_&_IIIT). Prof._Rakesh_Lodha,_Department_of_Pediatrics,_All_India_Institute_of_Medical_Sciences,_New_Delhi_(Hospital_Associated_Infections). Dr._Venkat_Panchagnula,_National_Chemical_Laboratory,_Pune_(Laser_Desorption_Ionization_Mass_Spectrometry).

 

Selected_Publications:

 

  1. Lahiri_P.,_Gogoi_P.,_and_Ghosh_D._(2023)_Single-Step_Capture_and_Targeted_Metabolomics_of_Alkyl-Quinolones_in_Outer_Membrane_Vesicles_(OMVs)_of_Pseudomonas_aeruginosa._Methods_Mol_Biol_2625,_201-2016.
  2. Pompilio_A,_Crocetta_V,_Ghosh_D_et_al._(2016)_Stenotrophomonas_maltophilia_phenotypic_and_genotypic_diversity_during_a_10-year_colonization_in_the_lungs_of_a_cystic_fibrosis_patient._Frontiers_in_Microbiology_2016;_7:_1551
  3. Pluháček_T,_Lemr_K,_Ghosh_D,_Milde_D,_Novák_J_and_Havlíček_V_(2016)_Characterization_of_Microbial_Siderophores_by_Mass_Spectrometry._Mass_spectrometry_Reviews._35:_35-47
  4. Ghosh_D.,_Salzman_NH,_Huttner_KM,_Paterson_Y,_Bevins_CL._Protection_against_enteric_salmonellosis_in_transgenic_mice_expressing_a_human_intestinal_defensin._Nature._422:522-6.Commentary:_Ganz_T._Microbiology:_Gut_defence._Nature._422:478-9.
  5. Ghosh_D.,_Porter_E,_Shen_B,_Lee_SK,_Wilk_D,_Drazba_J,_Yadav_SP,_Crabb_JW,_Ganz_T,_Bevins_CL._Paneth_cell_trypsin_is_the_processing_enzyme_for_human_defensin-5._Nat._Immunol._3:583-590._Commentary:_Zasloff_M._Trypsin,_for_the_defense._Nat_Immunol._2002_Jun;3(6):508-10.

 

Recent_Peer_Reviewed_Journals/Books

  1. Ghosh,_D._(2011)._Pathogenesis_of_Malabsorption_Syndrome:_Issues_on_Gut_Flora_and_Innate_Immunity._In:_Ghoshal,_U_Malabsorption_Syndrome_in_Tropics._Delhi:_Elsevier._179-204.
  2. Ghosh,_D._(2010)._Probiotics_and_Intestinal_Defensins:_Augmenting_the_First_Line_of_Defense_in_Gastrointentinal_Immunity._In:_Nair,_G._B._and_Takeda,_Y._Probiotic_Foods_in_Health_and_Disease._Delhi:_Oxford_&_IBH_Publishing_Co._61-74

 

Patents_(if_any)

  1. Selective_Detection_and_Analysis_of_Small_Molecules_Ghosh_D.,_Dharware_D._and_Panchagnula_V._Jawaharlal_Nehru_University,_New_Delhi_and_Central_Scientific_and_Industrial_Research_(CSIR),_New_Delhi._(Ind)_407/DEL/2011;_(EPO)_EP2676287A2_;_(USPTO)_20130323849A1._Technology_Ready_for_Transfer.

  Number_of_students_awarded/submitted_Ph._D.:_1

Number_of_Ph._D._students_currently_enrolled:_4